Background & Objectives: :Contact Dermatitis (C.D) is a common condition and 5.4% of population are suffering from it. Main treatments are based on emollients and corticosteroids. and in C.D cellular infiltration are mononuclears chiefly T. helper lymphocytes. The aim of this study was to have a new approach to herbals with new science covering and study the efficacy of alcoholic extracts of 4 herbals: henna, camomile cedar and aloevera in experimental C.D on mice.

  Methods: In a pilot experimental study first we chose 70 mice (ENMARY race, 50 ± 10gr weight as case group, then we shaved behind of their left ears (at the days 0 and 1) and rubbed DNCB 400 ^mg/_ml on the skin.At the 10th and 14th day the same amount of DNCB again was rubbed on the left ear. On the right ear the same amount of olive oil in Acetone dissolver was rubbed,as control group. Then at the times of 2, 10, 24, 48, 72, 96, 120, 150 hrs after the 2nd contact, the mice were killed with Ether, cutting the ears from base and prepared pathological sections for study of cellular infiltration. In second stage alcoholic extracts of 4 plants were prepared, then herbal cream with 4% concentration in cold cream as pallish cream were made, with considering the results of first stage that showed the maximal infiltration was at 120 hr after contact, the creams were rubbed every 12hr for 7 days on 40 mice in 4 groups that had been sensitized before with DNCB. 2 groups (20 mice) were considered as control (10 mice received no drugs and 10 mice received cold cream). 60 mice were chosen in the second phase and in general 130 mice were investigated.

  Results: lymphocytic infiltration reduced after 7 days application of drugs for camomile group about 66/7%, cedar 50%, alaevera 12/5% and henna 0%

  Conclusion: Camomile chammazolen, Inositol had the best response (66/7% severe response) on improvement of contact dermatitis (P=0.02)