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:: Volume 24, Issue 1 (Spring 2024) ::
J Ardabil Univ Med Sci 2024, 24(1): 107-119 Back to browse issues page
The Comparative Study of Antibacterial Effects of Different Hydroethanolic Extracts of Artemisia Species against Common Bacterial Strains in Nosocomial Infections
Ramin Abiri , Samira Ghasemi , Nastaran Sharei , Masumeh Shahbazi , Ahmad Emami , Mahdi Mojarrab *
Pharmaceutical Sciences Research Center, Research Institute for Health, Kermanshah University of Medical Sciences, Kermanshah, Iran , mmojarrab@kums.ac.ir
Abstract:   (177 Views)
Background: Nosocomial infections are considered as the important parts of the treatment challenges in hospitals. The genus Artemisia is widely distributed in Iran. Their species produce antibacterial, antiviral, and antifungal compounds belonging to different groups including phenols, terpenoids, sterols and polyacetylenes. The purpose of the present study is to investigate the in vitro effects of different hydroethanolic extracts of Artemisia species against bacterial strains in nosocomial infection.
Methods: 12 different extracts, including 50% and 70% hydroethanolic extracts were prepared from the aerial parts of Artemisia ciniformis, A. turanica, A. kopetdaghensis, A. khorasanica, A. vulgaris, and A. sieberi. The winterization of the extracts produced corresponding defatted extracts. The minimum inhibitory concentration (MIC) values of the extracts against the bacterial strains Staphylococcus aureus, Streptococcus pyogenes, Acinetobacter sp., Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococus epidermidis, Micrococcus luteus, Klebsiella  pneumoniae and Escherichia coli were measured using the microdilution broth method. Total phenolic contents were determined by the Folin-Ciocalteu method.
Results: Among the extracts analyzed in this experiment, the lowest MIC value was observed for the 50% hydroethanolic extract of A. turanica (0.25 mg/ml) against S. epidermidis. The largest range of bacterial sensitivity (6 strains) was related to the 50% hydroethanolic extracts of A. turanica (defatted and non-defatted) and A. kopetdaghensis (defatted). The growth of S. epidermidis was inhibited by all of the extracts. The highest total phenolic content and yield of extraction were recorded for 70% hydroethanolic extract of A. sieberi and 50% hydroethanolic extract of A. ciniformis, respectively.
Conclusion: The 50% hydroethanolic extract of A. turanica was superior to the other extracts in terms of the in vitro antibacterial spectrum and selective potency. A. turanica and A. kopetdaghensis are probably suitable choices for further phytochemical and antibacterial investigations.
Article number: 8
Keywords: Artemisia, Anti-bacterial Agents, Nosocomial Infections
Full-Text [PDF 472 kb]   (105 Downloads)    
Type of Study: short communication | Subject: General
Received: 2023/12/18 | Accepted: 2024/03/28 | Published: 2024/08/5
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Abiri R, Ghasemi S, Sharei N, Shahbazi M, Emami A, Mojarrab M. The Comparative Study of Antibacterial Effects of Different Hydroethanolic Extracts of Artemisia Species against Common Bacterial Strains in Nosocomial Infections. J Ardabil Univ Med Sci 2024; 24 (1) : 8
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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 24, Issue 1 (Spring 2024) Back to browse issues page
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