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:: Volume 18, Issue 1 (spring 2018) ::
J Ardabil Univ Med Sci 2018, 18(1): 120-130 Back to browse issues page
Effect of Curcumin on Bax, Bcl-2, Antioxidant Enzymes and Lipid Peroxidation of Sperm after Freezing Procedure
Taktam Sadat Vafa , Mojdeh Emadi , Seyed Damoon Sadoughi
Young Researchers and Elite Club, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Abstract:   (6371 Views)
Background & objectives: Curcumin has antioxidant properties. The aim of this study was to determine the effect of curcumin on bax, bcl-2, antioxidant enzymes and lipid peroxidation of sperm after freezing procedure.
Methods: In this experimental study, semen samples were collected from four mature Holstein bulls, twice a week in eight innings. Semen samples were divided into four groups. Zero (control), 10 (Experimental group one), 20 (Experimental group two) and 30 (Experimental group three) mg/ml of curcumin with diluents were added to the semen samples. After thawing, Bax, Bcl-2 and malondialdehyde levels as well as superoxide dismutase, glutathione peroxidase and catalase enzymes were measured in sperm samples using ELISA.
Results: According to the results, Bcl-2, superoxide dismutase, glutathione peroxidase and catalase levels  in sperm samples treated with 20 and 30 mg/ml curcumin significantly increased and Bax and malondialdehyde levels significantly decreased compared to control groups (p<0.05). This difference was not significant for sperm samples treated with 10 mg/ml curcumin.
Conclusion: Dose-dependent administration of curcumin decreased oxidative stress and lipid peroxidation and increased anti-apoptosis proteins in freeze-thawing sperms.
Keywords: Apoptosis, Antioxidant Enzymes, Sperm, Lipid Peroxidation, Curcumin
Full-Text [PDF 136 kb]   (1816 Downloads)    
Type of Study: article | Subject: General
Received: 2017/10/23 | Accepted: 2018/03/11 | Published: 2018/03/29
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Vafa T S, Emadi M, Sadoughi S D. Effect of Curcumin on Bax, Bcl-2, Antioxidant Enzymes and Lipid Peroxidation of Sperm after Freezing Procedure . J Ardabil Univ Med Sci 2018; 18 (1) :120-130
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Volume 18, Issue 1 (spring 2018) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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