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:: Volume 18, Issue 1 (spring 2018) ::
J Ardabil Univ Med Sci 2018, 18(1): 120-130 Back to browse issues page
Effect of Curcumin on Bax, Bcl-2, Antioxidant Enzymes and Lipid Peroxidation of Sperm after Freezing Procedure
Taktam Sadat Vafa , Mojdeh Emadi , Seyed Damoon Sadoughi
Young Researchers and Elite Club, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Abstract:   (395 Views)
Background & objectives: Curcumin has antioxidant properties. The aim of this study was to determine the effect of curcumin on bax, bcl-2, antioxidant enzymes and lipid peroxidation of sperm after freezing procedure.
Methods: In this experimental study, semen samples were collected from four mature Holstein bulls, twice a week in eight innings. Semen samples were divided into four groups. Zero (control), 10 (Experimental group one), 20 (Experimental group two) and 30 (Experimental group three) mg/ml of curcumin with diluents were added to the semen samples. After thawing, Bax, Bcl-2 and malondialdehyde levels as well as superoxide dismutase, glutathione peroxidase and catalase enzymes were measured in sperm samples using ELISA.
Results: According to the results, Bcl-2, superoxide dismutase, glutathione peroxidase and catalase levels  in sperm samples treated with 20 and 30 mg/ml curcumin significantly increased and Bax and malondialdehyde levels significantly decreased compared to control groups (p<0.05). This difference was not significant for sperm samples treated with 10 mg/ml curcumin.
Conclusion: Dose-dependent administration of curcumin decreased oxidative stress and lipid peroxidation and increased anti-apoptosis proteins in freeze-thawing sperms.
Keywords: Apoptosis, Antioxidant Enzymes, Sperm, Lipid Peroxidation, Curcumin
Full-Text [PDF 136 kb]   (95 Downloads)    
Type of Study: article | Subject: General
Received: 2017/10/23 | Accepted: 2018/03/11 | Published: 2018/03/29
1. Fraser L, Wysocki P, Ciereszko A, Plucienniczak G, Kotlowska M, Kordan W, et al. Application of biochemical markers for identification of biological properties of animal semen. Reprod Biol. 2006; 6(1): 5-20.
2. Purdy PH. A review on goat sperm cryopreservation. Small Rumin Res. 2006 Jun; 63(3): 215-25. [DOI:10.1016/j.smallrumres.2005.02.015]
3. Vilela CG, Marquez JM, Graham JK, Barfield JP. Cryopreservation of bison epididymal sperm: A strategy for improving post-thaw quality when collecting sperm in field conditions. Theriogenology. 2017 Feb; 89: 155-61. [DOI:10.1016/j.theriogenology.2016.09.044]
4. Fang Y, Zhong R, Chen L, Feng C, Sun H, Zhou D. Effects of astaxanthin supplementation on the sperm quality and antioxidant capacity of ram semen during liquid storage. Small Rumin Res. 2015; 130 Sep: 178-82. [DOI:10.1016/j.smallrumres.2015.05.016]
5. Sarıozkan S, Bucak MN, Tuncer PB, Buyukleblebici S, Canturk F. Influence of various antioxidants added to TCM-199 on post-thaw bovine sperm parameters, DNA integrity and fertilizing ability. Cryobiology. 2014 Feb. 68(1): 129-33. [DOI:10.1016/j.cryobiol.2014.01.007]
6. Sarıozkan S, Bucak MN, Tuncer PB, Büyükleblebici S, Eken A, Akay C. Influence of fetuin and hyaluronan on the post-thaw quality and fertilizing ability of Holstein bull semen. Cryobiology. 2015 Aug; 71(1): 119-24. [DOI:10.1016/j.cryobiol.2015.04.011]
7. Kim S, Agca C, Agca Y. Changes in rat spermatozoa function after cooling, cryopreservation and centrifugation processes. Cryobiology. 2012 Dec; 65(3): 215-23. [DOI:10.1016/j.cryobiol.2012.06.006]
8. Zhao Y, Buhr MM. Cryopreservation extenders affect calcium flux in bovine spermatozoa during a temperature challenge. J Androl. 1995 Jun; 16(3): 278-85.
9. Nair SJ, Brar AS, Ahuja CS, Sangha SP, Chaudhary KC. A comparative study on lipid peroxidation, activities of antioxidant enzymes and viability of cattle and buffalo bull spermatozoa during storage at refrigeration temperature. Anim Reprod Sci. 2006 Nov; 96(1-2): 21-9. [DOI:10.1016/j.anireprosci.2005.11.002]
10. Laulier C, Lopez BS. The secret life of Bcl-2: apoptosis-independent inhibition of DNA repair by Bcl-2 family members. Mutat Res. 2012 Oct-Dec; 751(2): 247-57. [DOI:10.1016/j.mrrev.2012.05.002]
11. Maes ME, Schlamp CL, Nickells RW. BAX to basics: How the BCL2 gene family controls the death of retinal ganglion cells. Prog Retin Eye Res. 2017 Mar; 57: 1-25. [DOI:10.1016/j.preteyeres.2017.01.002]
12. Renault TT, Dejean LM, Manon S. A brewing understanding of the regulation of Bax function by Bcl-xL and Bcl-2. Mech Ageing Dev. 2017 Jan; 161(Pt B): 201-10.
13. Franco R, Sánchez-Olea R, Reyes-Reyes EM, Panayiotidis MI. Environmental toxicity, oxidative stress and apoptosis: ménage à trois. Mutat Res. 2009 Mar; 674(1-2): 3-22. [DOI:10.1016/j.mrgentox.2008.11.012]
14. Chatterjee S, Gagnon C. Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing. Mol Reprod Dev. 2001 Aug; 59(4): 451-8. [DOI:10.1002/mrd.1052]
15. Yue HX, Li P, Jiang M, Lin L, Xu KH. Influence of cryopreservation with glycerol and freezing-thawing producer on the motility of human sperm. Natl J Androl. 2005 Mar; 11(3): 204-6.
16. Bandaya MN, Lonea FA, Rasoola F, Rashida M, Shikarib A. Use of antioxidants reduce lipid peroxidation and improve quality of crossbred ram sperm during its cryopreservation. Cryobiology. 2017 Feb; 74: 25-30. [DOI:10.1016/j.cryobiol.2016.12.008]
17. Baiee FH, Wahid H, Rosnina Y, Ariff O, Yimer N, Jeber Z, et al. Impact of Eurycoma longifolia extract on DNA integrity, lipid peroxidation, and functional parameters in chilled and cryopreserved bull sperm. Cryobiology. 2018 Feb; 80: 43-50. [DOI:10.1016/j.cryobiol.2017.12.006]
18. Maheshwari RK, Singh AK, Gaddipati J, Srimal RC. Multiple biological activities of curcumin. Life Sci. 2006 Mar; 78(18): 2081-7. [DOI:10.1016/j.lfs.2005.12.007]
19. Kant V, Gopal A, Pathak NN, Kumar P, Tandan SK, Kumar D. Antioxidant and anti-inflammatory potential of curcumin accelerated the cutaneous wound healing in streptozotocin-induced diabetic rats. Int Immunopharmacol. 2014 Jun; 20(2): 322-30. [DOI:10.1016/j.intimp.2014.03.009]
20. Roghani Dehkordi F, Roghani M, Baluchnejadmojarad T. The effect of curcumin on serum level of aspartate and alanine aminotransferase and cardiac level of oxidative stress markers in diabetic rats. pajoohande. 2012 Apr; 17(1): 18-25. [Full Text in Persian]
21. Kowluru RA, Kanwar M. Effects of curcumin on retinal oxidative stress and inflammation in diabetes. Nutr Metab. 2007 Apr; 4: 8. [DOI:10.1186/1743-7075-4-8]
22. Gil J, Lundeheim N, Soderquist L, Rodriguez-Martinez H. Influence of extender, temperature, and addition of glycerol on post-thaw sperm parameters in ram semen. Theriogenology. 2003 Mar; 59(5-6): 1241-55. [DOI:10.1016/S0093-691X(02)01177-9]
23. Ibănescu I, Leiding C, Ciornei ŞG, Roșca P, Sfartz I, Drugociu D. Differences in CASA output according to the chamber type when analyzing frozen-thawed bull sperm. Anim Reprod Sci. 2016 Mar; 166: 72-9. [DOI:10.1016/j.anireprosci.2016.01.005]
24. Ashrafi I, Kohram H, Tayefi-Nasrabadi H. Antioxidant effects of bovine serum albumin on kinetics, microscopic and oxidative characters of cryopreserved bull spermatozoa. Span J Agric Res. 2013 Jul; 11(3): 695-701. [Full Text in Persian] [DOI:10.5424/sjar/2013113-3870]
25. Shahbazzadeh R, Daghigh-Kia H, Moghaddam G, Dehghan G, Hosseinkhani A, Ashrafi I. Effect of different levels of Satureja sahendica alcoholic extract on the quality of freeze-thawed Holstein bull spermatozoa. J Anim Sci Res (Agric Sci). 2015 Spring; 25(1): 13-24. [Full Text in Persian]
26. Farhadi R, Daghigh-Kia H, Hosseinkhani A, Ghasemi Panahi B, Dehghan G, Ashrafi I. Effect of Origanum vulgare ethanol extract on quality parameters and malondialdehyde concentration of cryopreserved Holstein bull sperm. J Anim Sci Res (Agric Sci). 2015 Spring; 25(1): 1-11. [Full Text in Persian]
27. Malek-Mohammadi R, Roghani M, Salami M. The effect of aqueous extracts of Melissa officinalis on the oxidative stress indices in the midbrain tissue. Feyz. 2015 Apr; 19(1): 8-14. [Full Text in Persian]
28. Partyka A, Lukaszewicz E, Niżański W. Effect of cryopreservation on sperm parameters, lipid peroxidation and antioxidant enzymes activity in fowl semen. Theriogenology. 2012 May; 77(8): 1497-504. [DOI:10.1016/j.theriogenology.2011.11.006]
29. Dhami AJ, Kodagali SB. Freezability, enzyme leakage and fertility of buffalo spermatozoa in relation to the quality of semen ejaculates and extenders. Theriogenology. 1990 Nov; 34(5): 853-63. [DOI:10.1016/0093-691X(90)90557-A]
30. Liu T, Gao J, Zhou N, Mo M, Wang X, Zhang X, et al. The effect of two cryopreservation methods on human sperm DNA damage. Cryobiology. 2016 Jun; 72(3): 210-5. [DOI:10.1016/j.cryobiol.2016.04.004]
31. Chen YK, Liu QH, Li J, Xiao ZZ, Xu SH, Shi XH, et al. 2010. Effect of long-term cryopreservation on physiological characteristics, antioxidant activities and lipid peroxidation of red seabream (Pagrus major) sperm. Cryobiology. 2010 Oct; 61(2): 189-93. [DOI:10.1016/j.cryobiol.2010.07.003]
32. Guthrie HD, Welch GR. Effects of reactive oxygen species on sperm function. Theriogenology. 2012 Nov; 78(8): 1700-8. [DOI:10.1016/j.theriogenology.2012.05.002]
33. Osipova VP, Berberova NT, Gazzaeva RA, Kudryavtsev KV. Application of new phenolic antioxidants for cryopreservation of sturgeon sperm. Cryobiology. 2016 Feb; 72(2):112-118. [DOI:10.1016/j.cryobiol.2016.02.006]
34. Zargari M, Ahmadi S, Shabani S, Mahrooz A. Protective Effect of Curcumin on the Superoxide Dismutase and Catalase Activity in Kidney of Acetaminophen-exposed Rats. J Mazand Univ Med Sci. 2013 Feb; 22(97): 74-83. [Full Text in Persian]
35. Momeni H, Eskandari N. Effect of curcumin on kidney histopathological changes, lipid peroxidation and total antioxidant capacity of serum in sodium arsenite-treated mice. Exp Toxicol Pathol. 2016 Nov; 69(2): 93-7. [DOI:10.1016/j.etp.2016.08.006]
36. Mazani M, Tutunchi S, Shahi D, Manafi H, Yazdi M, Khajoie Najad M, et al. Prevention effect of turmeric extract on methotrexate-induced intestinal toxicity by alleviating oxidative stress in rats. J Urmia Univ Med Sci. 2014 Apr; 25(2): 119-28. [Full Text in Persian]
37. Calabrese V, Bates TE, Mancuso C, Cornelius C, Ventimiglia B, Cambria MT, et al. Curcumin and the cellular stress response in free radical-related diseases. Mol Nutr Food Res. 2008 Sep; 52(9): 1062-73. [DOI:10.1002/mnfr.200700316]
38. Pari L, Tewas D, Eckel J. Role of curcumin in health and disease. Arch Physiol Biochem. 2008 Jan; 114(2): 127-49. [DOI:10.1080/13813450802033958]
39. Momeni HR, Chehrei S, Atabaki Z, Eskandari N. Study of the effect of curcumin on sperm parameters dysfunction induced by cadmium in mice. Pejouhandeh. 2015 Jun; 20(2): 54-62. [Full Text in Persian]
40. Roghani M, Baluchnejadmojarad T. Antinociceptive Effect of Curcumin, an Effective Constituent of Turmeric, in Diabetic Rats and Evaluation of the Involvement of Lipid Peroxidation. Modares J Med Sci Pathol. 2012 Spring; 15(1): 23-32. [Full Text in Persian]
41. Soetikno V, Watanabe K, Sari FR, Harima M, Thandavarayan RA, Veeraveedu PT, et al. Curcumin attenuates diabetic nephropathy by inhibiting PKC-α and PKC-β1 activity in streptozotocin-induced type I diabetic rats. Mol Nutr Food Res. 2011 Nov; 55(11): 1655-65. [DOI:10.1002/mnfr.201100080]
42. Amouoghli Tabrizi B, Mohajeri D. Protective effect of edible turmeric (Curcuma longa Linn.) powder on early hepatic injury in diabetic rats. Feyz. 2010 Jan; 14(3): 190-99. [Full Text in Persian]
43. Hosseinzadeh S, Dabidi Roshan V. Effects of Curcumin supplementation on BDNF and Oxidative/antioxidative process in rat's hippocampus which exposed to lead. J Gorgan Univ Med Sci. 2010 Summer; 13(2): 1-8. [Full Text in Persian]
44. Khodaparast Z, yousofi AR, khoshvagti A. Investigation of Curcumin effects on liver tissue in adult male rats treated with cyclophosphamide. J Fasa Univ Med Sci. 2014 Sep; 4(3): 344-52. [Full Text in Persian]
45. Momeni HR, Darbandi N, Hoseini N, Jamshidi R. Protective effect of Curcumin and Curcuma longa extract on apoptosis of motor neurons in cultured spinal cord slices of adult mouse. Physiol Pharmacol. 2014 Spring; 18(1): 72-81. [Full Text in Persian]
46. Hamzavi Jahromi Z, Zolghadri Jahromi S, Hemayatkhah Jahromi V, Kargar Jahromi H, Erfanian S. Protective effect of Curcumin agains gamma-radiation on testis of Rats. HMJ. 2014 Jun; 18(2): 121-131. [Full Text in Persian]
47. Tvrdáa E, Tušimováb E, Kováčika A, Paálc D, Greifováa H, Abdramanovd A, et al. Curcumin has protective and antioxidant properties on bull spermatozoa subjected to induced oxidative stress. Anim Reprod Sci. 2016 Sep; 172: 10-20. [DOI:10.1016/j.anireprosci.2016.06.008]
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Vafa T S, Emadi M, Sadoughi S D. Effect of Curcumin on Bax, Bcl-2, Antioxidant Enzymes and Lipid Peroxidation of Sperm after Freezing Procedure . J Ardabil Univ Med Sci. 2018; 18 (1) :120-130
URL: http://jarums.arums.ac.ir/article-1-1532-en.html

Volume 18, Issue 1 (spring 2018) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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