[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 18, Issue 1 (spring 2018) ::
J Ardabil Univ Med Sci 2018, 18(1): 7-20 Back to browse issues page
A Cellular Perspective on the Importance of Oxidative Stress Effects on Sperm
Niloofar Sadeghi , Marzieh Tavalaee , Mohammad Hosein Nasr- Esfahani
Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Abstract:   (6242 Views)
Infertility, especially in the last decade, has been rising as a global problem, affecting approximately 10-15% of the world's population. The abundance and origin of various types of infertility are different. Studies have shown that reactive oxygen species (ROS) are involved in infertility, in particular in male infertility. Although ROS is essential for normal physiological functions of sperm such as capacitation, hyper activation, acrosome reactions, and binding to the oocyte, excessive levels can be one of the main causes of defective sperm function, which not only impairs the health of sperm DNA, but also affects fertilization via oxidation of proteins, in particular the fatty acids of the sperm cell membrane. Also, the elevated ROS levels facilitate sperm DNA damage, which leads to activation of apoptotic pathway and cell death. Therefore, the quality of semen is functionally reduced. Since the oxidative damage to sperm DNA is associated with both miscarriage and development abnormalities in the offspring, it is essential to find new strategies to recognize the cellular and molecular biology of sperm. Therefore, considering different mechanisms of oxidative stress affecting sperm can contribute significantly to the etiology of male infertility
Keywords: ROS, RNS, DNA Damage, Infertility, Antioxidant
Full-Text [PDF 301 kb]   (2829 Downloads)    
Type of Study: review article | Subject: General
Received: 2017/10/23 | Accepted: 2018/02/19 | Published: 2018/03/29
1. Sharma R, Biedenharn KR, Fedor JM, Agarwal A. Lifestyle factors and reproductive health: taking control of your fertility. Reprod Biol Endocrinol. 2013 Jul; 11(1): 66.‌ [DOI:10.1186/1477-7827-11-66]
2. Buzadzic B, Vucetic M, Jankovic A, Stancic A, Korac A, Korac B, et al. New insights into male (in) fertility: the importance of NO. Br J Pharmacol. 2015 Mar; 172(6): 1455-1467.‌ [DOI:10.1111/bph.12675]
3. Aitken RJ, Smith TB, Jobling MS, Baker MA, De Iuliis GN. Oxidative stress and male reproductive health. Asian J Androl. 2014 Jan; 16(1): 31.‌ [DOI:10.4103/1008-682X.122203]
4. Iwasaki A, Gagnon C. Formation of reactive oxygen species in spermatozoa of infertile patients. Fertil Steril. 1992 Feb; 57(2): 409-416.‌ [DOI:10.1016/S0015-0282(16)54855-9]
5. De Lamirande E, Gagnon C. Impact of reactive oxygen species on spermatozoa: a balancing act between beneficial and detrimental effects. Hum Reprod. 1995 Oct; 10(suppl_1): 15-21.‌‌
6. Agarwal A, Said TM, Bedaiwy MA, Banerjee J, Alvarez JG. Oxidative stress in an assisted reproductive techniques setting. Fertil Steril. 2006 Sep; 86(3): 503-512.‌ [DOI:10.1016/j.fertnstert.2006.02.088]
7. Tremellen K. Oxidative stress and male infertility—a clinical perspective. Hum Reprod Update. 2008 May-Jun; 14(3): 243-258.‌ [DOI:10.1093/humupd/dmn004]
8. Kumar TR, Doreswamy K, Shrilatha B. Oxidative stress associated DNA damage in testis of mice: induction of abnormal sperms and effects on fertility. Mutat Res. 2002 Jan; 513(1-2): 103-111.‌ [DOI:10.1016/S1383-5718(01)00300-X]
9. Athayde KS, Cocuzza M, Agarwal A, Krajcir N, Lucon AM, Srougi M, et al. Development of normal reference values for seminal reactive oxygen species and their correlation with leukocytes and semen parameters in a fertile population. J Androl. 2007 Jul-Aug; 28(4): 613-620.‌ [DOI:10.2164/jandrol.106.001966]
10. Agarwal A, Sharma RK, Sharma R, Assidi M, Abuzenadah AM, Alshahrani S, et al. Characterizing semen parameters and their association with reactive oxygen species in infertile men. Reprod Biol Endocrinol. 2014 May;12: 33.‌ [DOI:10.1186/1477-7827-12-33]
11. Ko EY, Sabanegh ES, Agarwal A. Male infertility testing: reactive oxygen species and antioxidant capacity. Fertil Steril. 2014 Dec; 102(6): 1518-1527.‌ [DOI:10.1016/j.fertnstert.2014.10.020]
12. Whittington K, Harrison SC, Williams KM, Day JL, Mclaughlin EA, Hull MG, et al. Reactive oxygen species (ROS) production and the outcome of diagnostic tests of sperm function. Int J Androl. 1999 Aug; 22(4): 236-242.‌ [DOI:10.1046/j.1365-2605.1999.00174.x]
13. Pasqualotto FF, Sharma RK, Nelson DR, Thomas AJ, Agarwal A. Relationship between oxidative stress, semen characteristics, and clinical diagnosis in men undergoing infertility investigation. Fertil Steril. 2000 Mar; 73(3): 459-464. [DOI:10.1016/S0015-0282(99)00567-1]
14. Dawson EB, Harris WA, Teter MC, Powell LC. Effect of ascorbic acid supplementation on the sperm quality of smokers. Fertil Steril. 1992 Nov; 58(5): 1034-1039.‌‌‌ [DOI:10.1016/S0015-0282(16)55456-9]
15. Kessopoulou E, Powers HJ, Sharma KK, Pearson MJ, Russell JM, Cooke ID, et al. A double-blind randomized placebo cross-over controlled trial using the antioxidant vitamin E to treat reactive oxygen species associated male infertility. Fertil Steril. 1995 Oct; 64(4): 825-831.‌ [DOI:10.1016/S0015-0282(16)57861-3]
16. Hughes CM, Lewis SE, McKelvey-Martin VJ, Thompson W. The effects of antioxidant supplementation during Percoll preparation on human sperm DNA integrity. Hum Reprod. 1998 May; 13(5): 1240-1247.‌ [DOI:10.1093/humrep/13.5.1240]
17. Rolf C, Cooper TG, Yeung CH, Nieschlag E. Antioxidant treatment of patients with asthenozoospermia or moderate oligoasthenozoospermia with high-dose vitamin C and vitamin E: a randomized, placebo-controlled, double-blind study. Hum Reprod. 1999 Apr; 14(4): 1028-1033.‌ [DOI:10.1093/humrep/14.4.1028]
18. Doshi SB, Khullar K, Sharma RK, Agarwal A. Role of reactive nitrogen species in male infertility. Reprod Biol Endocrinol. 2012 Dec; 15;10:109.
19. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007 Aug; 39(1): 44-84.‌ [DOI:10.1016/j.biocel.2006.07.001]
20. Aitken RJ, Clarkson JS. Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. J Reprod Fertil. 1987 Nov; 81(2): 459-469. [DOI:10.1530/jrf.0.0810459]
21. Sharma RK, Agarwal A. Role of reactive oxygen species in male infertility. Urology. 1996 Dec; 48(6): 835-850. [DOI:10.1016/S0090-4295(96)00313-5]
22. Rivlin J, Mendel J, Rubinstein S, Etkovitz N, Breitbart H. Role of hydrogen peroxide in sperm capacitation and acrosome reaction. Biol Reprod. 2004 Feb; 70(2): 518-522.‌ [DOI:10.1095/biolreprod.103.020487]
23. Koppers AJ, De Iuliis GN, Finnie JM, McLaughlin EA, Aitken RJ. Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa. J Clin Endocrinol Metab. 2008 Aug; 93(8): 3199-3207.‌ [DOI:10.1210/jc.2007-2616]
24. Vernet P, Aitken RJ, Drevet JR. Antioxidant strategies in the epididymis. Mol Cell Endocrinol.2004 Mar; 216(1-2): 31-39.‌ [DOI:10.1016/j.mce.2003.10.069]
25. Starkov AA. The role of mitochondria in reactive oxygen species metabolism and signaling. Ann N Y Acad Sci. 2008 Dec; 1147: 37-52.‌ [DOI:10.1196/annals.1427.015]
26. Wright C, Milne S, Leeson H. Sperm DNA damage caused by oxidative stress: modifiable clinical, lifestyle and nutritional factors in male infertility. Reprod Biomed Online. 2014 Jun; 28(6): 684-703.‌ [DOI:10.1016/j.rbmo.2014.02.004]
27. Rengan AK, Agarwal A, van der Linde M, du Plessis SS. An investigation of excess residual cytoplasm in human spermatozoa and its distinction from the cytoplasmic droplet. Reprod Biol Endocrinol. 2012 Nov 17; 10: 92.‌ [DOI:10.1186/1477-7827-10-92]
28. Choudhary R, Chawala VK, Soni ND, Kumar J, Vyas RK. Oxidative stress and role of antioxidants in male infertility. Pak J Physiol. 2010; 6(2): 54-59.‌
29. Whittington K. Relative contribution of leukocytes and of spermatozoa to reactive oxygen species production in human sperm suspensions. Int J Androl. 1999 Aug; 22(4): 229-235.‌ [DOI:10.1046/j.1365-2605.1999.00173.x]
30. Aziz N, Agarwal A, Lewis-Jones I, Sharma RK, Thomas AJ. Novel associations between specific sperm morphological defects and leukocytospermia. Fertil Steril. 2004 Sep; 82(3): 621-627.‌ [DOI:10.1016/j.fertnstert.2004.02.112]
31. Moskovtsev SI, Willis J, White J, Mullen JBM. Leukocytospermia: relationship to sperm deoxyribonucleic acid integrity in patients evaluated for male factor infertility. Fertil Steril.2007 Sep; 88(3): 737-740. [DOI:10.1016/j.fertnstert.2006.11.132]
32. Will MA, Swain J, Fode M, Sonksen J, Christman GM, Ohl D. The great debate: varicocele treatment and impact on fertility. Fertil Steril. 2011 Mar; 95(3): 841-852.‌ [DOI:10.1016/j.fertnstert.2011.01.002]
33. Tavalaee M, Bahreinian M, Barekat F, Abbasi H, Nasr-Esfahani MH. Effect of varicocelectomy on sperm functional characteristics and DNA methylation. Andrologia. 2015 Oct; 47(8):904-9.
34. Bahreinian M, Tavalaee M, Abbasi H, Kiani-Esfahani A, Shiravi AH, Nasr-Esfahani MH. DNA hypomethylation predisposes sperm to DNA damage in individuals with varicocele. Syst Biol Reprod Med. 2015 Mar; 61(4):179-86. [DOI:10.3109/19396368.2015.1020116]
35. Shiraishi K, Matsuyama H, Takihara H. Pathophysiology of varicocele in male infertility in the era of assisted reproductive technology. Int J Urol. 2012 Jun; 19(6): 538-550.‌ [DOI:10.1111/j.1442-2042.2012.02982.x]
36. Nasr-Esfahani MH, Salehi M, Razavi S, Anjomshoa M, Rozbahani S, Moulavi F, et al. Effect of sperm DNA damage and sperm protamine deficiency on fertilization and embryo development post-ICSI. Reprod Biomed Online. 2005 Aug; 11(2):198-205. [DOI:10.1016/S1472-6483(10)60959-5]
37. Durairajanayagam D, Agarwal A, Ong C. Causes, effects and molecular mechanisms of testicular heat stress. Reprod Biomed Online. 2015 Jan; 30(1):14-27. [DOI:10.1016/j.rbmo.2014.09.018]
38. Agarwal A, Deepinder F, Sharma RK, Ranga G, Li J. Effect of cell phone usage on semen analysis in men attending infertility clinic: an observational study. Fertil Steril. 2008 Jan; 89(1): 124-128.‌ [DOI:10.1016/j.fertnstert.2007.01.166]
39. Mc Gill JJ, Agarwal A. The impact of cell phone, laptop computer, and microwave oven usage on male fertility. Male Infertility. 2014; 161-77.
40. De Iuliis GN, Newey RJ, King BV, Aitken RJ. Mobile phone radiation induces reactive oxygen species production and DNA damage in human spermatozoa in vitro. PLoS One. 2009 Jul; 4(7): e6446. [DOI:10.1371/journal.pone.0006446]
41. Desai NR, Kesari KK, Agarwal A. Pathophysiology of cell phone radiation: oxidative stress and carcinogenesis with focus on male reproductive system. Reprod Biol Endocrinol. 2009 Oct 22; 7:114.‌ [DOI:10.1186/1477-7827-7-114]
42. Valavanidis A, Vlachogianni T, Fiotakis K. Tobacco smoke: involvement of reactive oxygen species and stable free radicals in mechanisms of oxidative damage, carcinogenesis and synergistic effects with other respirable particles. Int J Environ Res Public Health. 2009 Feb; 6(2): 445-462.‌ [DOI:10.3390/ijerph6020445]
43. Hengstler JG, Bolm-Audorff U, Faldum A, Janssen K, Reifenrath M, Götte W, et al. Occupational exposure to heavy metals: DNA damage induction and DNA repair inhibition prove co-exposures to cadmium, cobalt and lead as more dangerous than hitherto expected. Carcinogenesis. 2003 Jan; 24(1): 63-73.‌ [DOI:10.1093/carcin/24.1.63]
44. Kiziler AR, Aydemir B, Onaran I, Alici B, Ozkara H, Gulyasar T, et al. High levels of cadmium and lead in seminal fluid and blood of smoking men are associated with high oxidative stress and damage in infertile subjects. Biol Trace Elem Res. 2007 Winter; 120(1-3): 82-91.‌ [DOI:10.1007/s12011-007-8020-8]
45. Das M, Al-Hathal N, San-Gabriel M, Phillips S, Kadoch IJ, Bissonnette F, et al. High prevalence of isolated sperm DNA damage in infertile men with advanced paternal age. J Assist Reprod Genet. 2013 Jun; 30(6): 843-848.‌ [DOI:10.1007/s10815-013-0015-0]
46. Belloc S, Hazout A, Zini A, Merviel P, Cabry R, Chahine H, et al. How to overcome male infertility after 40: Influence of paternal age on fertility. Maturitas, 2014 May; 78(1): 22-29.‌ [DOI:10.1016/j.maturitas.2014.02.011]
47. Desai N, Sabanegh E, Kim T, Agarwal A. Free radical theory of aging: implications in male infertility. Urology. 2010 Jan; 75(1): 14-19.‌ [DOI:10.1016/j.urology.2009.05.025]
48. Kasahara E, Miyoshi M, Konaka R, Hiramoto K, Sasaki J, Tokuda M, et al. Role of oxidative stress in germ cell apoptosis induced by di (2-ethylhexyl) phthalate. Biochem J. 2002 Aug; 365(3): 849-856.‌ [DOI:10.1042/bj20020254]
49. Aitken RJ, Gordon E, Harkiss D, Twigg JP, Milne P, Jennings Z, et al. Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa. Biol Reprod. 1998 Nov; 59(5): 1037-1046.‌ [DOI:10.1095/biolreprod59.5.1037]
50. Agarwal A, Sekhon LH. The role of antioxidant therapy in the treatment of male infertility. Hum Fertil. 2010 Dec; 13:217–25. [DOI:10.3109/14647273.2010.532279]
51. Aitken RJ, Paterson M, Fisher H, Buckingham DW, Van Duin M. Redox regulation of tyrosine phosphorylation in human spermatozoa and its role in the control of human sperm function. J Cell Sci. 1995 May; 108(5): 2017-2025.‌
52. Gomez E, Irvine DS, Aitken RJ. Evaluation of a spectrophotometric assay for the measurement of malondialdehyde and 4-hydroxy-alkenals in human spermatozoa: Relationships with semen quality and sperm function. Int J Androl. 1998 Apr; 21(2): 81-94.‌‌ [DOI:10.1046/j.1365-2605.1998.00106.x]
53. Christova Y, James PS, Jones R. Lipid diffusion in sperm plasma membranes exposed to peroxidative injury from oxygen free radicals. Mol Reprod Dev. 2004 Jul; 68(3): 365-372.‌ [DOI:10.1002/mrd.20084]
54. Aitken RJ, Gibb Z, Baker MA, Drevet J, Gharagozloo P. Causes and consequences of oxidative stress in spermatozoa. Reprod Fertil Dev. 2016; 28(1-2): 1-10.‌ [DOI:10.1071/RD15325]
55. Moazamian R, Polhemus A, Connaughton H, Fraser B, Whiting S, Gharagozloo P, et al. Oxidative stress and human spermatozoa: diagnostic and functional significance of aldehydes generated as a result of lipid peroxidation. Mol Hum Reprod. 2015 Jun; 21(6): 502-515.‌ [DOI:10.1093/molehr/gav014]
56. Sanocka D, Kurpisz M. Reactive oxygen species and sperm cells. Reprod Biol Endocrinol. 2004 Mar; 23(2):12. [DOI:10.1186/1477-7827-2-12]
57. Razavi S, Nasr-Esfahani MH, Mardani M, Mafi A, Moghdam A. Effect of human sperm chromatin anomalies on fertilization outcome post-ICSI. Andrologia. 2003 Aug; 35(4): 238-43. [DOI:10.1046/j.1439-0272.2003.00566.x]
58. Nasr-Esfahani MH, Razavi S, Mozdarani H, Mardani M, Azvagi H. Relationship between protamine deficiency with fertilization rate and incidence of sperm premature chromosomal condensation post-ICSI. Andrologia. 2004 Jun; 36(3): 95-100. [DOI:10.1111/j.1439-0272.2004.00612.x]
59. Tavalaee M, Deemeh MR, Arbabian M, Kiyani A, Nasr-Esfahani MH. Relationship between fertilization rate and early apoptosis in sperm population of infertile individuals. Andrologia. 2014 Feb; 46(1): 36-41. [DOI:10.1111/and.12038]
60. Nasr-Esfahani MH, Naghshizadian N, Imani H, Razavi S, Mardani M, Kazemi S, et al. Can sperm protamine deficiency induce sperm premature chromosomal condensation? Andrologia. 2006 Jun; 38(3): 92-8. [DOI:10.1111/j.1439-0272.2006.00721.x]
61. Tavalaee M, Razavi S, Nasr-Esfahani MH. Influence of sperm chromatin anomalies on assisted reproductive technology outcome. Fertil Steril. 2009 Apr; 91(4): 1119-26. [DOI:10.1016/j.fertnstert.2008.01.063]
62. Zribi N, Chakroun NF, Elleuch H, Abdallah FB, Hamida ASB, Gargouri J, et al. Sperm DNA fragmentation and oxidation are independent of malondialdheyde. Reprod Biol Endocrinol. 2011 Apr; 9: 47.‌ [DOI:10.1186/1477-7827-9-47]
63. González-Marín C, Gosálvez J, Roy R. Types, causes, detection and repair of DNA fragmentation in animal and human sperm cells. Int J Mol Sci. 2012 Oct; 13(11): 14026-14052. [DOI:10.3390/ijms131114026]
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sadeghi N, Tavalaee M, Nasr- Esfahani M H. A Cellular Perspective on the Importance of Oxidative Stress Effects on Sperm . J Ardabil Univ Med Sci 2018; 18 (1) :7-20
URL: http://jarums.arums.ac.ir/article-1-1530-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 18, Issue 1 (spring 2018) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
Persian site map - English site map - Created in 0.08 seconds with 30 queries by YEKTAWEB 4502