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:: Volume 18, Issue 1 (spring 2018) ::
J Ardabil Univ Med Sci 2018, 18(1): 62-69 Back to browse issues page
Comparison of the Diagnostic Method of Cultivation with Amsel Standard in the Diagnosis of Gardnerella vaginalis in Patients with Genital Infection
Golsom Sorazar , Habib Tavassoli, SHshla Farzi Poor , Behbod Jafari , Masoud Nemati Attar
Department of Microbiology, Faculty of Microbiology, Islamic Azad University, Ahar, Iran
Abstract:   (5716 Views)
Background & objectives: Vaginal microbial infections are common, and involvement of bacterial agents in genital infection is equal to that of fungal and protozoal agents. Gardnerella vaginalis is an organism that is often thought to play the most important role in bacterial vaginosis. The aim of this study was to compare the value of the diagnostic method of cultivation with Amsel standard in the diagnosis of Gardnerella Vaginalis infection in patients with genital tract infection.
Methods: A cross-sectional study was performed on 150 women aged 15-55 years who complained of vaginal discharge during the period of the study, Jan 2017 to July 2017, in Alawi Hospital in Ardabil and were examined by a clinical examination. For each of the patients, three of the four diagnostic criteria of Amsel, including homogeneous discharge, PH measurements and whiff test were performed, and if two of them were positive, a questionnaire containing general and clinical information was completed. Using three sterile swabs, samples were taken from vaginal discharge. The first swab was used for culture, the second swab for the whiff testing and the third swab for Papanicolaou staining and verifying the presence of clue cell in the vaginal smear sample as a fourth grade of Amsel to diagnose bacterial vaginosis. In general, if three of the four Amsel criteria were positive in one person, it was considered to be positive in terms of Amsel's standard.
Results: Of 150 participants, 21 were diagnosed with Gardnerella vaginalis infection, of which 14 cases (%66.6) had positive Gardnerella culture. All of 21 patients (%100) with Gardnerella vaginalis had clue cells in Pap smear. The pH of vaginal discharge of 20 samples was 4.5 (%95.23), 18 samples had positive Amine tests (%85.71) and 16 samples had homogeneous secretion (%76.19).
Conclusion: The results of this study showed that culture method in comparison with Amsel diagnostic criteria did not have sufficient accuracy to detect Gardnerella vaginalis infection. In addition, the culture method is costly and time consuming.
Keywords: Gardnerella vaginalis, Proprietary Cultivate Medium, Amsel
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Type of Study: article | Subject: General
Received: 2017/09/21 | Accepted: 2017/12/21 | Published: 2018/03/29
1. Beckmann CH, Ling F, Smith R, Barzansky B. Maternity and maternity. Philadelphia 15th ed: Lippincott Williams & Wilkins; 2014. p. 269-70.
2. Thorsen P, Jensen IP, Jeune B, Ebbesen N, Arpi M, Bremmelgaard A, et al. A small number of microorganisms associated with bacterial vaginosis may form a pathologic nucleus: a papillary-based microbiological study among 3396 pregnant women. Am J Obste Gynecol. 1998 Mar; 178 [3]: 580-7. [DOI:10.1016/S0002-9378(98)70442-9]
3. Molana Z, Ghazi Saeidi K, Frequency of GardenerllaVaginalis among pregnant and non-pregnant women referred to Babol University of Medical Sciences clinics in 1997. J GorganUniv Med Sci. 2000 Mar; 2 [1] :12-18.[ Full Text in Persian]
4. Asghari B,Jenabi Namin M. Henry Davidson Medical Microbiology. McPherson RA, Pincus MR. 1 st ed, Tehran Publisher: ArtinTeb, 2017: 42-168
5. Jafarnejad F, Nayeban S, Ghazvini K. DiagnosticValueof Amsel'sClinicalCriteriafor Diagnosis of Bacterial Vaginosis. IJOGI. 2017 Nov; 20 [9]: 1-7.
6. Shir Mohammadi M, Taghizadeh Z. Comprehensive Women's Diseases, 2 nd ed. Tehran: Andisheh Rafi Publications, 2016: 44-210
7. Ivanbagha R, Soroush Barhaghi M T, Babapour J, Fathi S. Risk factors and clinical findings of Gardnerella vaginalis infection in women referring to Tabriz health centers. JIATUMS. 2011 Autumn;21 [3]: 214-221. [full translation]
8. Rahimi G, Etehad G, Tazakori Z. The Prevalence of Bacterial Vaginosis in Women Referring to Health Centers. JHC. 2012 Spring; 13 [1]: 1-5.[ Full text in Persian]
9. Goodarzi F, Hosseini M, Hemmatzadeh F. Evaluation of Demographic and Clinical Characteristics of Patients with Bacterial Vaginosis in Ahwaz Amir Al Momenin Hospital. Journal of FasaUniversity of Medical Sciences. 2014Nov; 4 [2]: 218-24.[ Full text in Persian]
10. Uma S, Balakrishnan P, Muruqavel KG, Srikrishnan AK, Kumarasumy N, Anand S, et al. Bacterial vaginosis in women of low socioeconomic status living in slum areas in Chennai, India. Sex Health. 2006 Dec; 3 [4]: 297-8. [DOI:10.1071/SH06036]
11. Salehian M, Mozafari N, Forouhesh Tehrani H. Commercial Antibiotic resistance of Gardnerella vaginalis in patients with bacterial vaginosis versus 2 antibiotics metronidazole and clindamycin. Iran J Clinic Infect Dis; 2010 Autumn; 13 [42]:43- 47.[ Full text in Persian]
12. Thomason JL, Gelbart SM, Scaglione NJ. Bacterial vaginosis: current review with indications for asymptomatic therapy. Am J Obestet Gynecol. 1991 Oct; 165 [4 pt 2]: 1210-7. [DOI:10.1016/S0002-9378(12)90729-2]
13. Afrakhteh M, Mahdavi A. Bacterial vaginosis and urinary tract infection. IJOGI. 2006 summer: 9 [1]: 108-113. [ Full text in Persian]
14. Holley RL, Richter HE, Varner RE, Pair L, Schwebke JR. A randomized, double-blind clinical trial of vaginal acidification versus placebo for the treatment of symptomatic bacterial vaginosis. Sex Transm Dis. 2004 Apr; 31 [4]: 236-8. [DOI:10.1097/01.OLQ.0000118423.20985.E7]
15. Borjian S, Shojaei H, Shabanian M, Deris F. Diagnosis of Gardenella associated vaginosis in Borujen women's outpatient Clinic, 2000. J Shahrekord Univ Med Sci. 2002 Jan; 3 [4] :38-44.
16. Berek JS. Berek& Novak's Gynecology, 15th ed. Philadelphia: Lippincott Williams & Wilkins; 2014: 541-5.
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Sorazar G, Tavassoli H, Farzi Poor S, Jafari B, Nemati Attar M. Comparison of the Diagnostic Method of Cultivation with Amsel Standard in the Diagnosis of Gardnerella vaginalis in Patients with Genital Infection. J Ardabil Univ Med Sci 2018; 18 (1) :62-69
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Volume 18, Issue 1 (spring 2018) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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