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:: Volume 23, Issue 2 (summer 2023) ::
J Ardabil Univ Med Sci 2023, 23(2): 192-205 Back to browse issues page
Study of association between rs34674752 polymorphism in Sharpin gene and Alzheimer’s disease by Tetra ARMS PCR
Zahra Moeinara , Elham Siasi * , Robab Tabatabaeii
Department of Genetic, School of biology science, Islamic Azad University, North Tehran Branch, Tehran, Iran , emi_biotech2006@yahoo.ca
Abstract:   (617 Views)
Background & objective: The causes of Alzheimer's disease are currently unknown. Genetic and environmental factors can be effective in creating of this disease. In recent studies, one of the genes and its polymorphisms that was known to affect Alzheimer is SHARPIN. This study aimed to investigate the presence of rs34674752 polymorphism in the SHARPIN gene and its relation with Alzheimer's disease in the population of Iranian patients.
Methods: This study was performed on 50 people with Alzheimer's disease and 50 healthy controls. After blood sampling and DNA extraction, genotyping was done by Tetra ARMS PCR. The data was statistically analyzed.
Results: Results showed that the frequency of GG, GT and TT genotypes of rs34674752 polymorphism in control and patient groups was 100%, 0% and 0%, respectively. Both control and patient groups were in Hardy Weinberg equilibrium. There was no significant correlation between people's genotype and the possibility of Alzheimer's disease, and among the demographic factors, only the relationship between age group and the disease was significant (p=0.029).
Conclusion: According to the results of this study, there was no statistically significant association between the rs34674752 polymorphism in the SHARPIN gene and Alzheimer's disease in the studied Iranian population. To confirm the present study results, the investigation of populations with different societies and a larger quantity of samples are recommended.

 
Article number: 8
Keywords: Single Nucleotide Polymorphism, Alzheimer's Disease, Polymerase Chain Reaction
     
Type of Study: article | Subject: Genetics and molecular medicine
Received: 2023/06/2 | Accepted: 2023/08/6 | Published: 2023/09/11
References
1. Navipour E, Neamatshahi M, Barabadi Z, Neamatshahi M, Keykhosravi A. Epidemiology and risk factors of alzheimer's disease in Iran: A systematic review. Iran J Public Health. 2019; 48(12):2133-39. [Full text in Persian] [DOI:10.18502/ijph.v48i12.3544]
2. Mirzaei-Fini F, Dowlati MA, Dehghani Ashkezari M. Investigating the association of val/metpolymorphism of the BDNF gene with the incidence of disease in patients with alzheimer and comparison with healthy elderly people in Iran. Feyz J Kashan Uni Med Sci. 2018; 22:617-23. [Full text in Persian]
3. Bellenguez C, Küçükali   F, Jansen I, Kleineidam L, Moreno-Grau S, Ami N, et al. New insights into the genetic etiology of Alzheimer's disease and related dementias. Nat Genet. 2022; 54:412-36. [DOI:10.1038/s41588-022-01024-z] [PMID] []
4. Armstrong RA. Risk factors for Alzheimer's disease. Folia Neuropathol. 2019; 57(2):87-105. [DOI:10.5114/fn.2019.85929] [PMID]
5. Sims R, Hill M, Williams J. The multiplex model of the genetics of alzheimer's disease. Nat Neurosci. 2020; 23(3):311-322. [DOI:10.1038/s41593-020-0599-5] [PMID]
6. Trepso WL. Risk Factors for Alzheimer's disease. Sci Insigt. 2020; 32(2):125-32. [DOI:10.15354/si.20.re036]
7. Ferreira Silva MV, Loures CG, Vieira Alves LC, Cruz de Souza L, Gomes Borges KB, Graças Carvalho M. Alzheimer's disease: risk factors and potentially protective measures. J Biomed Sci. 2019; 26(33):1-11. [DOI:10.1186/s12929-019-0524-y] [PMID] []
8. Perkovic MN, Pivac N. Genetic markers of alzheimer's disease.
9. Adv Exp Med Biol. 2019; 1192:27-52.
10. Mintun MA, LO AC, Duggan Evans C, Wessels AM, Ardayfio PA, Andersen SW, Shcherbinin S, et al. Donanemab in early alzheimer's disease. N Engl J Med. 2021; 384:1691-704. [DOI:10.1056/NEJMoa2100708] [PMID]
11. Sahiner M, Yilmaz AS, Gungor B, Sahiner N. A review on photo-therapeutic approaches in alzheimer's disease. J Funct Biomater. 2023; 14:50. [DOI:10.3390/jfb14010050] [PMID] []
12. Asanomi Y, Shigemizu D, Akiyama SH, Miyashita A, Mitsumori R, Har N, et al. A functional variant of sharpin confers increased risk of late onset alzheimer's disease. J Hum Genet. 2022; 67:203-8. [DOI:10.1038/s10038-021-00987-x] [PMID] []
13. Asanomi Y, Shigemizu D, Miyashita A, Mitsumori R, Mori T, Hara N, et al. A rare functional variant of sharpin attenuates the inflammatory response and associates with increased risk of late-onset alzheimer's disease. Mol Med 2019; 25(20):1-9. [DOI:10.1186/s10020-019-0090-5] [PMID] []
14. Moreno-Grau S, de Rojas I, Hernández I, Quintela I, Montreal L, Alegret M, et al. Genome-wide association analysis of dementia and its clinical endophenotypes reveal novel loci associated with alzheimer's disease and three causality networks. Alzheimers Dement. 2019; 15(10):1333-47. [DOI:10.1016/j.jalz.2019.06.4950] [PMID]
15. Park JY, Lee D, Lee JJ, Gim J, Gunasekaran TI, Choi KY, et al. A missense variant in sharpin mediates alzheimer's disease specific brain damages. Transl Psychiatry. 2021; 11:590. [DOI:10.1038/s41398-021-01680-5] [PMID] []
16. Kamboh MI. Genomics and functional genomics of alzheimer's disease. Neurotherapeutics. 2022; 19:152-72. [DOI:10.1007/s13311-021-01152-0] [PMID] []
17. Soheili-Nezhad S, Jahanshad N, Guelfi S, Khosrowabadi R, Saykin AJ, Thompson P.M, et al. Imaging genomics discovery of a new risk variant for alzheimer's disease in the postsynaptic sharpin gene. Hum Brain Mapp. 2020; 41:3737-48. [DOI:10.1002/hbm.25083] [PMID] []
18. Yin J, Feng W, Yuan H, Yuan J, Wu L. Association analysis of polymorphisms in STARD6 and near ECHDC3 in alzheimer's disease patients carrying the apop ε4 allele. Neuropsychiatr Dis Treat. 2019; 15:213-18. [DOI:10.2147/NDT.S186705] [PMID] []
19. Esmaeili Anvar N, Bazazzadegan N, Ohadi M, Kamali K. Association between interleukin 16 gene polymorphisms (rs1131445, rs4072111) and late onset of alzheimer's disease in Iranian patients. Iranian J Age. 2016; 11(1):64-71. [DOI:10.21859/sija-110164]
20. Hampanezhad Y, Khalaj-Kondori M, Khodaei M, Talebi M. Frequency of rs2282649 and rs12285364 polymorphisms in SORL1 gene and their association with alzheimer's disease in Azari population in northwest of Iran. Sci J Kurd Uni Med Sci. 2020; 24:46-56. [Full text in Persian] [DOI:10.29252/sjku.24.6.46]
21. Kim YJ, Paik JW, Kang WS, Kim SK, Lee K. Genetic association of STARD6 polymorphisms with alzheimer's disease in a Korean population. J Neurol Sci. 2016; 366:100-1. [DOI:10.1016/j.jns.2016.05.013] [PMID]
22. Bossù P, Ciaramella A, Moro ML, Bellincampi L, Bernardini S, Federici G, et al. Interleukin-18 gene polymorphisms predict risk and outcome of alzheimer's disease. J Neurol Neurosurg Psychiatry. 2007; 78(8):807-11. [DOI:10.1136/jnnp.2006.103242] [PMID] []
23. Cordeiro Q, Noguti R, Bottino C, Vallad H. Study of association between genetic polymorphisms of phospholipase A2 enzymes and alzheimer's disease. Arq Neuropsiquiatr. 2010; 68(2):189-93. [DOI:10.1590/S0004-282X2010000200007] [PMID]
24. Desikan RS, Schork AJ, Wang Y, Thompson WK, Dehghan A, Ridker PM, et al. Polygenic overlap between C-reactive protein, plasma lipids, and alzheimer disease. Circulation. 2015; 131:2061-69. [DOI:10.1161/CIRCULATIONAHA.115.015489] [PMID] []
25. Andrade-Guerrero J, Santiago-Balmaseda A, Jeronimo-Aguilar P, Vargas-Rodríguez I, Cadena-Suárez AR, Sánchez-Garibay C, et al. Alzheimer's disease: An updated overview of Its genetics. Int J Mol Sci. 2023; 24(4):3754. [DOI:10.3390/ijms24043754] [PMID] []
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Moeinara Z, Siasi E, Tabatabaeii R. Study of association between rs34674752 polymorphism in Sharpin gene and Alzheimer’s disease by Tetra ARMS PCR. J Ardabil Univ Med Sci 2023; 23 (2) : 8
URL: http://jarums.arums.ac.ir/article-1-2285-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 23, Issue 2 (summer 2023) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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