Department of Sports Physiology, Faculty of Physical Education and Sports Sciences, University of Guilan, Rasht, Iran , farhmani2001@yahoo.com
Abstract: (830 Views)
Background & objectives: Dynamic disorders of mitochondria cause the pathogenesis of many diseases, such as type 2 diabetes. Therefore, the aim of this study was to investigate the interactive effect of aerobic exercises and atorvastatin consumption on the expression of MFN1/2 and DRP1 in hepatocytes of rat liver with type 2 diabetes. Methods:In this experimental study, 25 male rats were divided into 5 equal groups: diabetes, healthy control, persistence+diabetes, atorvastatin+diabetes, persistence+atorvastatin+ diabetes. Type 2 diabetes was induced by streptozotocin (STZ) in mice. The training groups performed the running program on the treadmill for eight weeks. Atorvastatin and atorvastatin-exercise groups received atorvastatin (10 mg/kg) by gavage. 48 hours after the last training session, the rats were dissected; their liver tissue was removed and immediately frozen in liquid nitrogen solution at a temperature of minus 80 ° C to measure MFN1/2 and DRP1. One-way analysis of variance and Tukey post hoc test were used for statistical analysis at a significance level of p<0.05. Results: The results showed that induction of type 2 diabetes decreased the expression of MFN1/2 and increased DRP1 compared to the healthy group. After eight weeks of intervention, a significant increase was observed in the expression level of MFN1 (p<0.05), but this increase was not significant in MFN2 and there was no significant difference in the expression of factors between the groups.Also, after eight weeks, a significant decrease in DRP1 gene expression was observed (p<0.05). This decrease was significant in comparison with the combined groups compared to the patient group. Conclusion: It is possible that a combination of aerobic exercises and atorvastatin may positively regulate the expression of genes related to mitochondrial dynamics in diabetes.
Zolfali Pourfar M, Rahmani Nia F, Farzanegi P. The Interaction Effect of Aerobic Exercise and Atorvastatin Consumption on the expression level of MFN1/2 and DRP1 in Hepatocytes of the Rat Liver with Type 2 Diabetes. J Ardabil Univ Med Sci 2022; 21 (4) : 3 URL: http://jarums.arums.ac.ir/article-1-2161-en.html