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Study of Klebsiella pneumoniae Vaccine Based on the Epitopes of Outer Membrane Proteins: An Immunoinformatic Approach
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Hamid Vaez *    , Mohammad Amin Sargazi , Ali Zeidabadi , Mohammadreza Javan , Farzad Khademi , Abbas Pishdadian |
| Department of Microbiology, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran , h_vaez@zbmu.ac.ir |
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Abstract: (62 Views) |
Background: Klebsiella pneumoniae (K. pneumoniae) infections are usually considered life-threatening. Vaccination plays a crucial role in reducing the spread and mortality of infectious diseases. The present study aimed to investigate outer membrane protein K36 (OmpK36) and outer membrane protein X (OmpX) to identify suitable B-cell and T-cell epitopes for vaccine development against K. pneumoniae infections.
Methods: To identify suitable B-cell and T-cell epitopes, bioinformatics servers were utilized, including VaxiJen, IEDB, APP, ABCpred, ExPASy, and EMBOSS. Molecular and physicochemical characteristics, as well as human similarity, toxicity, and allergenicity of epitopes were investigated.
Results: The results of this study revealed that OmpK36 and OmpX are immunogenic. In total, 18 epitopes were identified, 13 for OmpK36 and 5 for OmpX. Exclusion criteria were applied, and eight epitopes were ultimately selected.
Conclusion: According to the results of this study, eight appropriate epitopes for B cells and T cells were proposed for vaccine design against K. pneumoniae. Further in vitro and in vivo studies are recommended before determining the use of epitopes. |
Article number: 6 |
| Keywords: K. pneumonia, Bacterial Infections, Klebsiella Infections, Outer Membrane Proteins, Vaccine |
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Full-Text [PDF 1233 kb]
(45 Downloads)
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Type of Study: article |
Subject:
Microbiology
Received: 2025/07/10 | Accepted: 2025/09/13 | Published: 2025/09/27
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Ethics code: IR.ZBMU.REC.1403.174
Vaez H, Sargazi M A, Zeidabadi A, Javan M, Khademi F, Pishdadian A. Study of Klebsiella pneumoniae Vaccine Based on the Epitopes of Outer Membrane Proteins: An Immunoinformatic Approach. J Ardabil Univ Med Sci 2025; 25 (1) : 6 URL: http://jarums.arums.ac.ir/article-1-2517-en.html
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