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Investigating the Expression level of miRNA-21, miRNA-155, miRNA-182, and miRNA-437 along with Inflammatory Markers in Cerebrospinal Fluid of Patients with Multiple Sclerosis
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Behrouz Shademan    , Masoud Nikanfar , Jafar Rezaei , Mehdi Hassanpour , Mohammad Nouri , Alireza Nourazarian * |
| Department of Basic Medical Sciences, Khoy University of Medical Sciences, Khoy, Iran , noorazarian_a@khoyums.ac.ir |
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Abstract: (809 Views) |
Background & objectives: Evidence suggests that microRNAs (miRNAs) are essential for immune cell differentiation and function. In addition, miRNAs play an essential role in regulating the expression of pro-inflammatory genes in neurons. Therefore, we investigated the relationship between miRNA expression and inflammatory markers in the CSF of patients with multiple sclerosis.
Methods: RT-PCR analysis was performed on CSF samples from patients with multiple sclerosis (MS) and a control group to measure the expression level of miRNA-21, miRNA-155, miRNA-182, and miRNA-437. In addition, the levels of the inflammatory cytokines including IL-1β, IL-6, and TNF-α in CSF were measured using ELISA. A quantitative turbidimetric method was also used to measure high-sensitivity C-reactive protein (hs-CRP).
Results: A significant difference was found in the expression level of miRNAs and inflammatory factors in the CSF of patients with MS compared with the control group (p<0.05). The results of receiver operating characteristic (ROC) analysis showed the area under the curve for miRNA-21 (AUC=0.97, p<0.0001), miRNA-182 (AUC=0.97, p<0.0001), and miRNA-155 (AUC=0.96, p<0.0001). The miRNA-155 level in CSF played a very important role in the accurate diagnosis of MS. Significant correlations were found between inflammatory cytokines and miRNA-21, miRNA-155, and miRNA-182, as well as an indirect and moderate correlation between miRNA-437 and hs-CRP.
Conclusion: In MS patients, CSF levels of IL-1, IL-6, TNF-α, hs-CRP, and selected miRNAs can be used as biomarkers of CNS inflammation and neurodegenerative processes.
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Article number: 6 |
| Keywords: Multiple Sclerosis (MS), MicroRNAs, Inflammatory Markers, Receiver Operating Characteristics (ROC) |
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Full-Text [PDF 1090 kb]
(341 Downloads)
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Type of Study: article |
Subject:
neuroscience
Received: 2022/10/1 | Accepted: 2022/11/15 | Published: 2022/07/1
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