[Home ] [Archive]   [ فارسی ]  
:: Main :: Current Issue :: Archive :: Search :: :: :: register ::
Main Menu
Home::
Editorial Board::
Editorial Policy::
For Authors::
For Reviewers::
Ethics::
Articles archive::
Registration::
Contact us::
::
..
Indexing

 

 

 

 

 

 
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Creative commons

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

..
:: Volume 19, Issue 1 (spring 2019) ::
J Ardabil Univ Med Sci 2019, 19(1): 7-19 Back to browse issues page
Hyperglycemia- Induced NF-κB Activation Increases microRNA-146a Expression in Human Umbilical Vein Endothelial Cells
Elnaz Salmani Korjan , Kamila Kamali , Majid Katebi , Afshin Samiei , Farhad Ghadiri Soufi *
Molecular Medicine Research Center, Health institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran. , dr.f.g.soufi@gmail.com
Abstract:   (5442 Views)
Background & objectives: Nuclear Factor kappa B (NF-κB), a master switch transcription factor, plays a critical role in the progression and development of hyperglycemia-induced microangiopathy. Hyperglycemia activates NF-κB, and subsequently increases pro-inflammatory cytokines such as TNF-α, IL-6 and IL-1β leading to development of inflammation. Some new studies have revealed the involvement of microRNA-146a (miR-146a) in the pathogenesis of diabetic complications through an NF-κB-dependent negative feedback loop manner. Despite numerous reports indicating changes of plasma miR-146a during hyperglycemia, the origin of this change remains unclear. This study was designed to evaluate the role of NF-κB on the miR-146a gene expression level in human umbilical vein endothelial cells (HUVECs) during a hyperglycemic condition.
Methods: HUVECs were cultured in normal glucose (5 mmol/L), and hyperglycemic (25 mmol/L) endothelial cell growth medium in the six well plates for 24 h. JSH-23 (30 μmol/L), as an inhibitor of NF-κB translocation to the nucleus, was added to the culture medium, 30 min before induction of hyperglycemia. Quantitative Real Time PCR was performed to measure the expression levels of miR-146a and mRNA NF-κB. NF-κB activity was measured by Elisa.
Results: Hyperglycemia markedly increased the NF-κB activity and mRNA level in HUVECs. The expression of miR-146a significantly increased in hyperglycemic group compared to the normoglycemic group. On the other hand, JSH-23 prevented from miR-146a increment in hyperglycemic group and also it increased the mRNA expression level of NF-κB in this group.
Conclusion: This result shows that NF-κB increases the gene expression of miRNA-146a in the early phase of hyperglycemia in HUVECs.
 
Keywords: Nuclear Factor- κB, Hyperglycemia, HUVECs, miR-146a
Full-Text [PDF 230 kb]   (2805 Downloads)    
Type of Study: article | Subject: فیزیولوژی
Received: 2018/12/21 | Accepted: 2019/03/11 | Published: 2019/03/30
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Salmani Korjan E, Kamali K, Katebi M, Samiei A, Ghadiri Soufi F. Hyperglycemia- Induced NF-κB Activation Increases microRNA-146a Expression in Human Umbilical Vein Endothelial Cells . J Ardabil Univ Med Sci 2019; 19 (1) :7-19
URL: http://jarums.arums.ac.ir/article-1-1649-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 19, Issue 1 (spring 2019) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
Persian site map - English site map - Created in 0.07 seconds with 41 queries by YEKTAWEB 4623