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:: Volume 23, Issue 3 (Autumn 2023) ::
J Ardabil Univ Med Sci 2023, 23(3): 292-308 Back to browse issues page
A Novel Design of a Non-Enzymatic Biosensor for the Detection of Hydrogen Peroxide Based on MWCNT/Co3O4/Hemoglobin Structures
Masoomeh Saboorifar , Ali Shamsazar , Asadollah Asadi * , Mostafa Shourian
Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran , asady@uma.ac.ir
Abstract:   (585 Views)
Background: Determining the concentration of hydrogen peroxide in liquids and biological samples is very important because of its effects on human health. This study aimed to design a new electrochemical biosensor based on hemoglobin to detect hydrogen peroxide in serum samples.
Methods: In this study, a basic science, a biosensor based on modifying the glassy carbon electrode surface with a nanocomposite consisting of cobalt oxide nanoparticles and multi-walled carbon nanotube functionalized with a carboxyl group (MWCNT/Co3O4) and hemoglobin stabilized on this nanocomposite was made as a biological recognition element.
Results: In optimal conditions, the biosensor was used to measure different concentrations of hydrogen peroxide. The designed biosensor showed a wide linear response range from 10 μM to 500 μM, a detection limit of 0.512 μM, and high reproducibility and stability.
Conclusion: In this innovative research work, MWCNTs/Co3O4 nanocomposite was used to make a diagnostic biosensor. The presented biosensor showed an acceptable performance in the measurement of hydrogen peroxide in serum samples and laboratory solutions.

 
Article number: 7
Keywords: Biosensor, Nanocomposite, Hemoglobin, Hydrogen Peroxide
Full-Text [PDF 771 kb]   (214 Downloads)    
Type of Study: article | Subject: Biochemistry
Received: 2023/09/26 | Accepted: 2023/11/28 | Published: 2023/12/17
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Saboorifar M, Shamsazar A, Asadi A, Shourian M. A Novel Design of a Non-Enzymatic Biosensor for the Detection of Hydrogen Peroxide Based on MWCNT/Co3O4/Hemoglobin Structures. J Ardabil Univ Med Sci 2023; 23 (3) : 7
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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 23, Issue 3 (Autumn 2023) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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