[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 15, Issue 2 (summer 2015) ::
J Ardabil Univ Med Sci 2015, 15(2): 171-178 Back to browse issues page
Isolation of CD34 Positive Hair Follicle Stem Cells Using Magnetic Activating Cell Sorting (MACS) Method
Sh Heydari Tajaddod, N Najafzadeh , M Mahdavi Rad, H Sheikhkanloui Milan, H Kalarestaghi, V Nejati
Abstract:   (5017 Views)

  Background & objectives: Hair follicle stem cells (HFSCs) are multipotent and various types of HFSCs were introduced. HFSCs separation using cell surface markers is one of the interesting strategies in the replacement of old methods. In this study, we used magnetic activating cell sorting (MACS) to separate HFSCs.

  Methods: In this study, HFSCs were isolated from Balb/c mice and dissected under an invert microscopy, and bulge area isolated and the bulge cells cultivated about 14 days. The CD34 positive cells isolated using CD34 monoclonal antibody and magnetic activated cell sorting system (MACS), then the cells incubated in DMEM/F12 and 10% FBS. The CD34 positive cells counted using a neubauer slide and evaluated under a fluorescent microscopy.

  Results: Here, we isolated CD34 positive cells using MACS and 12±1. 04% of HFSCs were CD34 positive and we found that, CD34 positive cells survived during 7 days cell culture in vitro.

  Conclusion: The results show that MACS is useful to increasing density gradient of cells in vitro.

Keywords: Hair Follicle Stem Cells, Magnetic Activating Cell Sorting, CD34, Fluorescent Microscopy
Full-Text [PDF 164 kb]   (1625 Downloads)    
Type of Study: article |
Received: 2015/01/15 | Accepted: 2015/05/13 | Published: 2015/07/1
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Heydari Tajaddod S, Najafzadeh N, Mahdavi Rad M, Sheikhkanloui Milan H, Kalarestaghi H, Nejati V. Isolation of CD34 Positive Hair Follicle Stem Cells Using Magnetic Activating Cell Sorting (MACS) Method. J Ardabil Univ Med Sci. 2015; 15 (2) :171-178
URL: http://jarums.arums.ac.ir/article-1-850-en.html


Volume 15, Issue 2 (summer 2015) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
Persian site map - English site map - Created in 0.1 seconds with 31 queries by YEKTAWEB 3986