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:: Volume 15, Issue 2 (summer 2015) ::
J Ardabil Univ Med Sci 2015, 15(2): 144-153 Back to browse issues page
Molecular Detection of TEM-1 Type Extended-Spectrum ß-Lactamases in Enterobacteriaceae Isolated from Urinary Tract Infections in Ardabil, Iran
N Danesh far, H Peeri Dogaheh , M Ghiamirad
Abstract:   (5176 Views)

  Background & objectives: Resistant microbial strains are a serious threat to public health in different societies. A mong the extended-spectrum β-lactamases (ESBL) producing strains the Enterobacteriaceae family which is considered as the main factors producing urinary tract infections, have created many problems in treatment of this kind of infections. This study was conducted to determine the frequency of β-lactamase TEM-1 gene in Enterobacteriaceae isolated from urine samples in Ardabil city.

  Methods: Within 6 months, 400 urinary isolates of Enterobacteriaceae of inpatients and outpatients were collected in Ardabil hospitals and were identified by standard methods. Antimicrobial susceptibility of isolates was tested by disk diffusion method, and ESBL producer confirmatory test was conducted using combined disk. Finally, the frequency of β-lactamase TEM-1 gene in producing extended-spectrum β-lactamases strains was investigated using PCR.

  Results: From 400 isolates of Enterobacteriaceae, 150 cases (37.5%) were ESBL producing. PCR results showed presence of the TEM-1 gene in 69 cases (46%). The frequency of this gene in isolates of Enterobacter (Aerogenes, Cloacae), Klebsiella (Pneumoniae, Oxytoca) and E. coli was obtained to be 62.5%, 54.5% and 44.8%, respectively. Proteus mirabilis and Serratia marcescens strains were lacking these genotypes.

  Conclusion: As regards the presence of TEM-1 gene, there is also increasing in other members of the Enterobacteriaceae family including Klebsiella and Enterobacter in addition to E. coli, therefore sufficient identification of this strains is necessary to prescribe the right medicine.

Keywords: Extended-Spectrum Beta-Lactamases, TEM-1, Entrobacteriaceae, Ardabil
Full-Text [PDF 187 kb]   (1083 Downloads)    
Type of Study: article |
Received: 2015/01/15 | Accepted: 2015/06/2 | Published: 2015/07/1
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Danesh far N, Peeri Dogaheh H, Ghiamirad M. Molecular Detection of TEM-1 Type Extended-Spectrum ß-Lactamases in Enterobacteriaceae Isolated from Urinary Tract Infections in Ardabil, Iran. J Ardabil Univ Med Sci. 2015; 15 (2) :144-153
URL: http://jarums.arums.ac.ir/article-1-848-en.html


Volume 15, Issue 2 (summer 2015) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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