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:: Volume 20, Issue 1 (spring 2020) ::
J Ardabil Univ Med Sci 2020, 20(1): 94-103 Back to browse issues page
The Effect of Different Concentrations of Smyrnium cordifolium (F: Apiaceae) Extract on Apoptosis and Proliferation of MCF-7
Leila Soltani , Maryam Darbemamieh
Department of Animal Sciences, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran
Abstract:   (89 Views)
 
Introduction & objectives: Application of traditional medicine and identification of herbs to treat cancer are being on the rise. Little information is available on the anticancer effects of Smyrnium cordifolium bioss species. For this purpose, the present study investigated the cytotoxic and apoptotic effects of the alcoholic extract of S. cordifulium.
Methods: After preparing the plant and its alcoholic extract, different concentrations of the extract (0, 2, 10, 50 and 250 μg/ml) were added to the culture medium of MCF-7 cells. MTT assay was used to evaluate cytotoxicity of different extract concentrations. In addition, acridine orange-ethidium bromide staining was used to assess apoptosis rates. Data was analyzed by SPSS software at the significance level of 5%.
Results: the results of this study showed that S. cordifolium extract at 250μg/ml concentration had a more inhibitory effect on proliferation compared to other treatment groups. Moreover, this concentration (250μg/ml) had a significant effect on apoptosis in comparison with other concentrations.
Conclusion: In conclusion, it seems that alcoholic extract of S. cordifolium can partially reduce proliferation of cancer cells.
 
Keywords: Smyrnium cordifolium, Cancer, MCF-7 Cell Line, Alcoholic Extract, Cytotoxicity
Full-Text [PDF 314 kb]   (36 Downloads)    
Type of Study: article | Subject: هماتولوژی و انکولوژی
Received: 2020/04/15 | Accepted: 2020/08/1 | Published: 2020/09/22
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Soltani L, Darbemamieh M. The Effect of Different Concentrations of Smyrnium cordifolium (F: Apiaceae) Extract on Apoptosis and Proliferation of MCF-7. J Ardabil Univ Med Sci. 2020; 20 (1) :94-103
URL: http://jarums.arums.ac.ir/article-1-1842-en.html


Volume 20, Issue 1 (spring 2020) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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