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:: Volume 18, Issue 1 (spring 2018) ::
J Ardabil Univ Med Sci 2018, 18(1): 80-90 Back to browse issues page
FNDC5 Gene Expression and Irisin Protein Level of Visceral Fat Tissue after Eight Weeks of Resistance Training in Type 2 Diabetic Rats
Ali Abdi , Nasrin Ramezani , Mehdi Amini
Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
Abstract:   (386 Views)
Background & objectives: Irisin is a novel myokine that encoded by FNDC5 gene and effects on obesity, metabolism and glucose homeostasis through browning of white adipose tissue and thermogenesis. The main purpose of this study was to investigate the changes of FNDC5 gene expression and Irisin protein level of visceral fat tissue after eight weeks of resistance training in type 2 diabetic rats.
Methods: Eighteen male Wistar rats (8 week old) were used for this study. Diabetes was induced using nicotinamide and streptozotocin . Five days after inducing diabetes, rats with fasting blood glucose levels between 127-600 mg / dl were selected as diabetic subjects. Rats were homogenized according to the body weight and assigned into two groups including control-diabetes (n=9) and resistance training-diabetes (n=9). Training group exercised resistance training for eight weeks (5 days a week). The resistance training protocol consisted of climbing   a one-meter- high ladder, with a weight attached to a tail sleeve. Quantitative Real time RT-PCR and ELISA Kit were used for assessment of expression level of FNDC5 gene and Irisin protein, respectively. Data were analyzed using independent t- test at p≤0.05.
Results: Resistance training significantly increased the expression level of FNDC5 gene and Irisin protein in visceral adipose tissue in type 2 diabetic rats.
Conclusion: It seems that FNDC5 gene and Irisin protein have an important role in metabolic diseases and can be affected by resistance training. Perhaps the changes in the levels of these metabolic indicators is a potential new target for the treatment of metabolic disorders, such as T2DM (type 2 diabetes).
Keywords: Resistance Training, Gene Expression FNDC5, Irisin, Type 2 Diabetes
Full-Text [PDF 199 kb]   (93 Downloads)    
Type of Study: article | Subject: General
Received: 2018/10/23 | Accepted: 2018/02/19 | Published: 2018/03/29
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Abdi A, Ramezani N, Amini M. FNDC5 Gene Expression and Irisin Protein Level of Visceral Fat Tissue after Eight Weeks of Resistance Training in Type 2 Diabetic Rats . J Ardabil Univ Med Sci. 2018; 18 (1) :80-90
URL: http://jarums.arums.ac.ir/article-1-1522-en.html

Volume 18, Issue 1 (spring 2018) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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