[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 17, Issue 4 (winter 2018) ::
J Ardabil Univ Med Sci 2018, 17(4): 456-464 Back to browse issues page
Evaluation of the Effect of Andrographolide on the Serum Activity of Superoxide Dismutase and Catalase Enzymes and Serum Levels of Malondialdehyde in the Rats with Secondary Iron Overload
Farid Manafi , Reza Alipanah-Moghadam , Ali Nemati , Vadood Malekzadeh
Department of Clinical Biochemistry, Faculty of Medicine, Ardabil University of Medical Sciences, , Ardabil, Iran
Abstract:   (1385 Views)
Background & objectives: Andrographolide has potent antioxidant properties. There is still not a complete understanding of the antioxidant effects of andrographolide. In this study, the effect of andrographolide on the serum antioxidant activity of superoxide dismutase and catalase was investigated in the rats with secondary iron overload. 
Methods: In this experimental study, 32 rats were divided into 4 groups of 8 each. The groups included: 1-Control 2- Receiving iron 3- Receiving iron treated with 7 mg/kg andrographolide 4- Receiving 7 mg/kg andrographolide. The serum activity of superoxide dismutase and catalase and serum level of malondialdehyde (MDA) was measured using colorimetric method. Prussian blue was used to evaluate iron deposition in liver.
Results: The results showed that iron was deposited in the liver. The activity of superoxide dismutase and catalase in the secondary iron receiving group, compared to control group, was significantly increased (p<0.05). The activity of catalase was significantly decreased in the group receiving iron and treated with andrographolide, compared to the control and iron receiving groups (p<0.05). Also, the activity of superoxide dismutase in the group receiving iron and treated with andrographolide decreased significantly compared to the iron receiving group (p<0.05). Serum levels of malondialdehyde in the iron receiving group were higher than the control group (p<0.05). Serum levels of malondialdehyde in the group receiving iron and treated with andrographolide, compared with the iron receiving group, showed a significant decrease (p<0.05).
Conclusion: Andrographolide (7mg/kg) reduced the serum activity of catalase and superoxide dismutase and malondialdehyde serum levels in rats with secondary iron overload.

 
 
Keywords: Andrographolide, Iron Overload, Superoxide Dismutase, Catalase, MDA.
Full-Text [PDF 1163 kb]   (399 Downloads)    
Type of Study: article | Subject: General
Received: 2017/06/20 | Accepted: 2017/12/21 | Published: 2017/12/31
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Manafi F, Alipanah-Moghadam R, Nemati A, Malekzadeh V. Evaluation of the Effect of Andrographolide on the Serum Activity of Superoxide Dismutase and Catalase Enzymes and Serum Levels of Malondialdehyde in the Rats with Secondary Iron Overload. J Ardabil Univ Med Sci. 2018; 17 (4) :456-464
URL: http://jarums.arums.ac.ir/article-1-1490-en.html


Volume 17, Issue 4 (winter 2018) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
Persian site map - English site map - Created in 0.1 seconds with 31 queries by YEKTAWEB 3749