[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 17, Issue 4 (winter 2018) ::
J Ardabil Univ Med Sci 2018, 17(4): 417-426 Back to browse issues page
Evaluation of miR-143 and miR-338 Expression Profiles in Tumor Tissue and Margin in Patients with Gastric Cancer
Mahsa Amini , Bahram Golestani Imani , Behzad Baradaran
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract:   (1538 Views)
 
Background & objectives: Micro-RNAs are non-coding RNAs with a length of approximately 22 nucleotides, which, by binding to the target gene's mRNA, regulate its expression and play an important role in tumor suppression. Changes in the expression level of microRNAs play a crucial role in the pathobiology of multiple cancers. In this study, the expression levels of miR-143 and miR-338 were compared in gastric cancer and its margin.
Methods: This case-control study was performed on 35 biopsy samples of gastric cancer and adjacent tissue of the patients who were admitted to Imam Reza Hospital. Total RNA was extracted from the tissue using Trizol reagent and based on the company's instructions. Then, the acquired microRNAs were used to synthetize cDNA. Expression of microRNAs was measured by RT-PCR. U6 was used as a house keeping gene. Statistically, the obtained results were analyzed using Graph pad Prism software.
Results: According to the results obtained in this study, the expression levels of miR-143 (p≤0.1244) and miR-338 (p≤0.0059)  in tumor tissue, compared to the adjacent tissue,  were down-regulated. Reduced expression of miR-143 and miR-338 in the tumor tissue, in comparison to margin tissue, was about four folds.
Conclusion: This study showed that the average expression level of miR-143 and miR-338 was significantly decreased in gastric cancer tissues compared to adjacent non-tumor tissues, and these results strongly suggest that miR-143 and miR-338 may play a key role in gastric cancer progression; therefore, they may be considered tumor markers.
Keywords: Gastric Cancer, micro RNA, miR-143, miR-338
Full-Text [PDF 456 kb]   (464 Downloads)    
Type of Study: article | Subject: General
Received: 2017/02/19 | Accepted: 2017/09/21 | Published: 2017/12/31
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Amini M, Golestani Imani B, Baradaran B. Evaluation of miR-143 and miR-338 Expression Profiles in Tumor Tissue and Margin in Patients with Gastric Cancer . J Ardabil Univ Med Sci. 2018; 17 (4) :417-426
URL: http://jarums.arums.ac.ir/article-1-1485-en.html


Volume 17, Issue 4 (winter 2018) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
Persian site map - English site map - Created in 0.1 seconds with 31 queries by YEKTAWEB 3785