:: Volume 16, Issue 2 (summer 2016) ::
J Ardabil Univ Med Sci 2016, 16(2): 159-168 Back to browse issues page
Apoptotic Effect of Specific siRNA against HMGA2 on PC3 Prostate Adenocarcinoma Cell Line
SHima Khajouee Ravari , Behzad Baradaran *
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran , behzad_im@yahoo.com
Abstract:   (8240 Views)

Background & objectives: Prostate cancer is one of the main reasons of death between men. Although there are many methods for treatment of this cancer but most of the patients still are died of the postoperative recurrence and metastasis of disease. Over expression of HMGA2 gene was observed in many human malignancies such as colorectal cancer, thyroid, pancreatic carcinoma and lung cancers. The aim of this study was to investigate the effect of HMGA2 specific small interfering RNAs (siRNAs) on viability and apoptosis in PC3 prostate adenocarcinoma cell line. 

Methods: siRNA transfection was performed with liposome approach. The cytotoxic effects of siRNA were determined using MTT assay on the PC3 cells and apoptosis was quantified using TUNEL assay.

Results: Transfection with siRNA significantly suppressed the expression of HMGA2 gene in dose dependent manner after 48 hours resulting in spontaneous apoptosis. Moreover, siRNA transfection had effects on prostate cancer cells viability.

Conclusion: Our results suggest that the HMGA2 specific siRNA effectively decreases prostate cancer cells viability and induces apoptosis in this cell line. Therefore it can be considered as a potent adjuvant in prostate cancer therapy.

Keywords: HMGA2, siRNA, PC3, Apoptosis, Cytotoxic
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Type of Study: article | Subject: General
Received: 2015/12/23 | Accepted: 2016/06/2 | Published: 2016/06/28

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Volume 16, Issue 2 (summer 2016) Back to browse issues page