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MicroRNA-143 Inhibits Migration of Human Prostate Cancer Cells (PC3)
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| Elshan Bazhan, Behzad Baradaran |
| Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran |
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Abstract: (8125 Views) |
Background & objectives: Prostate cancer is one the most common cancer in men whose incidence is increasing in many countries. According to the studies, decreased expression of miR-143 has been reported in prostate cancer. In this study, we replaced miRNA-143 in prostate cancer cells by vector based miRNA-143 and evaluated its inhibitory effects on migration of prostate cancer cells (PC3).
Methods: MTT assay was performed to reach an inhibitory concentration of Genticin antibiotic (G418 (in PC3 cells. Then, miRNA-143 vector was transfected into PC3 cells via JetPEI transfection reagent. The transfected cells were selected by G418 antibiotic according to a 2-week treatment with IC50 concentration. Then, the expression level of miRNA-143 was measured by qRT-PCR method. To evaluate the effect of miRNA-143 in inhibition of migration, scratch wound healing assay was performed.
Results: Results of MTT assay showed the IC50 level of G418 on PC3 cells was obtained to be 141.9 μg / ml. The results of qRT-PCR indicated increased expression of miRNA-143 in PC3 cells transfected with miRNA-143 compared to control cells. Finally, the results of wound healing assay showed migration reduction in transfected cells compared with control cells (empty vector).
Conclusion: The results showed that miRNA-143 play an important role in cell migration during prostate cancer metastasis, and it can be a good candidate for molecular treatments. |
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| Keywords: miRNA-143, Prostate Cancer, Metastasis, Transfection |
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Full-Text [PDF 1270 kb]
(2677 Downloads)
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Type of Study: article |
Subject:
General
Received: 2016/12/21 | Accepted: 2017/05/20 | Published: 2017/07/1
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