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:: Volume 19, Issue 1 (spring 2019) ::
J Ardabil Univ Med Sci 2019, 19(1): 30-40 Back to browse issues page
Drug Resistance Patterns and Genotyping of Acinetobacter baumannii Strains Isolated from Patients Admitted to Shahrekord Teaching Hospitals Using REP-PCR
Atefe Sarafan Sadeghi , Najmeh Ansari , Farzad Khademi , Reza Mir Nejad, Behnam Zamanzad
Emam Hosein Hospital, Esfahan University of Medical Sciences, Esfahan, Iran
Abstract:   (1198 Views)
Background & objectives: In recent years, Acinetobacter baumannii has been shown to be associated with several nosocomial infections, including pneumonia, bacteraemia, urinary tract infections, wound infection and meningitis. This organism can survive in the hospital environment and rapidly develops resistance to many antibiotics. The molecular genotyping can increase our knowledge about the spread of A. baumannii strains from one hospital to another and their drug resistance. Therefore, this study aimed to determine the prevalence of antibiotic resistance profile as well as phylogenetic relationships of A. baumannii strains in Shahrekord teaching hospitals.
Methods: In this study, antibacterial susceptibility patterns of A. baumannii strains isolated from different clinical specimens (urine, blood, sputum) to amikacin, ampicillin/sulbactam, aztreonam, cefepime, ceftazidime, ciprofloxacin, gentamycin, imipenem, meropenem, norfloxacin, ofloxacin, piperacillin/tazobactam, tobramycin were tested using disk diffusion )Kirby-Bauer( method. Finally, genotyping of A. baumannii strains was performed using REP-PCR method.
Results: During this study, 50 samples of patients were identified as A. baumannii) 71%(, and their drug resistance rates were assessed. All A. baumannii strains were resistant to ceftazidime and cefepime and also a high rate of resistance to aztreonam, norfloxacin, ciprofloxacin, amikacin, imipenem, gentamycin, and ampicillin-sulbactam were observed. On the other hand, our results demonstrated nine genotype groups among A. baumannii strains based on REP-PCR method.
Conclusion: Due to the high prevalence of antibiotic resistance among isolated A. baumannii strains, similarities between different genotypes and the dispersion of these genotypes in different parts of Shahrekord hospitals, the implementation of infection control programs in different parts of the hospital is necessary.
 
Keywords: Acinetobacter baumannii, Antibiotic Resistance, Genotyping, REP-PCR
Full-Text [PDF 234 kb]   (147 Downloads)    
Type of Study: article |
Received: 2018/12/21 | Accepted: 2019/03/11 | Published: 2019/03/30
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Sarafan Sadeghi A, ansari N, khademi F, Mir Nejad R, Zamanzad B. Drug Resistance Patterns and Genotyping of Acinetobacter baumannii Strains Isolated from Patients Admitted to Shahrekord Teaching Hospitals Using REP-PCR . J Ardabil Univ Med Sci. 2019; 19 (1) :30-40
URL: http://jarums.arums.ac.ir/article-1-1654-en.html


Volume 19, Issue 1 (spring 2019) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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