:: Volume 17, Issue 1 (spring 2017) ::
J Ardabil Univ Med Sci 2017, 17(1): 69-80 Back to browse issues page
Evaluation of Histopathological and Histomorphological Effects of Kombucha Extract (Camellia sinenesis) on Stomach Surgical Wound Healing in Adult Male Rat
Fariborz Moayer , Mohammad Abarkar, Hamed Ashrafzade
Abstract:   (4026 Views)

Background & Objectives: Wounds healing especially as to surgical wounds is considered clinical problem. Kombucha is used as a food and adminstarted for wound healing. The aim of this study was to assess histopathological and histomorphometrical effect of Kombucha on surgical wound healing process in stomach of rat.

Methods: Thirty adult male Wistar rats with 10-week-old and weighted between 200-250 g were randomly divided into two groups (Control and Experimenral). After anesthesia, rats’ stomach was removed and a gastrotomy incision with the length of 1 cm was made in greater curvature, thereafter, sutured in 2 layers. Experimental group received Kombucha extract (1 ml/100gbw) and control group received normal saline (1 ml/100gbw) daily for 14 days through the gavage. In 3, 7 and 14 days after operation, five rats of each group were euthanized and stomach tissue specimens were collected for histopatholocical study by H&E and Masson's trichrome staining. Histopathological and histomorphometrical studies of wound healing among the groups were carried out considering more effective agents on wound healing including proliferation of fibroblasts, angiogenesis, re-epithelialization and collagen organization in healing tissue.

Results: The numbers of fibroblasts, capillary buds and organized collagen content in healing site of experimental group were significantly more than control group (p<0.05). The size of epithelial gap in experimental group was significantly lesser than control group (p<0.05).

Conclusion: Based on the results, Kombucha extract possesses beneficial effects on healing of stomach surgical wounds in rats.

Keywords: Kombucha Tea, Stomach, Wound Healing, Rat
Full-Text [PDF 417 kb]   (1251 Downloads)    
Type of Study: article | Subject: General
Received: 2016/08/22 | Accepted: 2016/12/26 | Published: 2017/03/30


XML   Persian Abstract   Print



Volume 17, Issue 1 (spring 2017) Back to browse issues page