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:: Volume 13, Issue 1 (spring 2013) ::
J Ardabil Univ Med Sci 2013, 13(1): 86-92 Back to browse issues page
Determing the Prevalence Rate of Mycoplasma genitalium in Patients with Prostatitis by PCR-RFLP Technique
Gholamreza Irajian, Reza Mirnejad , Mohammad reza Jalali Nodoshan, Nafiseh Ghorbanpour
Abstract:   (6484 Views)

  Background & Objectives: Prostatitis is relatively one of the common diseases in elderly men. Treatment of this disease is difficult and because of frequent relapses, it provides complicated problems for patients and the physicians. Detection of reservoirs and determination of prevalence of involved microbes in prostatitis are important in the epidemiology and control of the disease. This study was conducted to determine the prevalence of Mycoplasma genitaliumin patients with prostatitis by sequencing and PCR-RFLP techniques.

  Methods : In this cross-sectional study, 200 paraffin-embedded prostate samples from patients with prostatitis during 2008-2011 were checked for M. gentialium. After cutting the tissues and homogenization, the genomic DNA was extracted and used as template in PCR. Primers targeting a 465 bp regions of 16S rRNA of M. genitalium were used in the assay. PCR products were sequenced and Cac8I, Bbs I, EcoRI, AluI, TaqI endonucleases were used in RFLP analysis.

  Results : Of 200 samples, 4 were positive in PCR. The results of DNA sequencing and RFLP confirmed the amplified genes corresponded to M. genitaliumG37.

  Conclusion : The Mycoplasma genome present in tissue samples of prostate showed this bacterium could be one of the risk factors for prostatitis in men. However large studies and control groups are needed to prove this finding.

Keywords: Prostatits; Mycoplasma genitalium; PCR-RFLP;16S rRNA
Full-Text [PDF 196 kb]   (1231 Downloads)    
Type of Study: article | Subject: Special
Received: 2011/12/15 | Accepted: 2013/01/1 | Published: 2013/03/21
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Irajian G, Mirnejad R, Jalali Nodoshan M R, Ghorbanpour N. Determing the Prevalence Rate of Mycoplasma genitalium in Patients with Prostatitis by PCR-RFLP Technique. J Ardabil Univ Med Sci. 2013; 13 (1) :86-92
URL: http://jarums.arums.ac.ir/article-1-130-en.html


Volume 13, Issue 1 (spring 2013) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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