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:: Volume 16, Issue 2 (summer 2016) ::
J Ardabil Univ Med Sci 2016, 16(2): 116-123 Back to browse issues page
Effect of QRDR Mutations on Ciprofloxacin Resistance in Clinical Isolates of Pseudomonas aeruginosa
Roqiyeh Nouri, Mohammad Ahangarzadeh Rezaee , Alka Hasani, Mohammad Aghazadeh, Mohammad Asgharzadeh, Morteza Ghojazadeh
Department of Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract:   (5088 Views)

Background & objectives: Fluoroquinolones have important role in treatment of P. aeruginosa infections. The main mechanism of fluoroquinolones resistance in P. aeruginosa is mutations in the quinolone-resistance-determining region (QRDR) of gyrA and parC genes. The aim of this study was to investigate the role of these mutations in ciprofloxacin resistance in different clinical isolates of P. aeruginosa.

Methods: A total of 75 clinical P. aeruginosa isolates were collected from different university-affiliated hospitals in Tabriz. Minimum inhibitory concentrations (MICs) of ciprofloxacin were evaluated by Etest assay. DNA sequences of the QRDR of gyrA and parC were determined by dideoxy chain termination method.

Results: From 75 isolates, 77.33% were resistant to ciprofloxacin. No amino acid changes were detected in gyrA or parC genes of the ciprofloxacin susceptible isolates. Thr-83→Ile substitution in gyrA was observed in all ciprofloxacin resistant isolates. About 90% of them had Ser-87→Leu substitution in parC. Geometric mean MICs of ciprofloxacin were different for various clinical isolates of P. aeruginosa which had the same situation in type and location of gyrA and parC mutations. Moreover, the geometric mean MIC in isolates from urine was significantly (p<0.05) higher than isolates from tracheal aspirates.

Conclusion: Mutations in gyrA and parC genes are the major mechanisms for ciprofloxacin resistance in clinical isolates of P. aeruginosa. Moreover, the role of different effective factors in fluoroquinolone resistance can be different in various clinical isolates of P. aeruginosa.

Keywords: Pseudomonas aeruginosa, Fluoroquinolone, Antibiotic Resistance, QRDR.
Full-Text [PDF 125 kb]   (1416 Downloads)    
Type of Study: article | Subject: General
Received: 2015/12/19 | Accepted: 2016/02/28 | Published: 2016/06/28
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Nouri R, Ahangarzadeh Rezaee M, Hasani A, Aghazadeh M, Asgharzadeh M, Ghojazadeh M. Effect of QRDR Mutations on Ciprofloxacin Resistance in Clinical Isolates of Pseudomonas aeruginosa. J Ardabil Univ Med Sci. 2016; 16 (2) :116-123
URL: http://jarums.arums.ac.ir/article-1-1092-en.html


Volume 16, Issue 2 (summer 2016) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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