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:: Volume 13, Issue 3 (autumn 2013) ::
J Ardabil Univ Med Sci 2013, 13(3): 305-316 Back to browse issues page
Screening of Missense SNPs in Coding Regions of COX-2 as a Key Enzyme Involved in Cancer
Sodabeh Jahanbakhsh-Godehkahriz , Elnaz Naderi, Ashraf Mohamadkhani
Abstract:   (4317 Views)

  Background & Objectives: Non-synonymous single nucleotide polymorphism (nsSNPs) which results in disruption of protein function are used as markers in linkage and association of human proteins that might be involved in diseases and cancers .

  Methods: To study the functional effect of nsSNP in cyclooxygenase-2 (COX2 ) amino acids, the nucleotide sequences encoding COX-2 gene in cancers were extracted from the NCBI (gi|223941909) data bank (283 cases) and analyzed by SIFT, I-Mutant 2.0, SNP and GO, PANTHER and FASTSNP servers. These servers involve programs that predict the effects of amino acid substitution on protein function, stability and missense .

  Results: COX-2 is an essential enzyme for the production of pro-inflammatory prostaglandins which are relevant to cancer development and progression. The substitutions in some positions such as R228H and S428A of COX-2 in most of cancers linked to reformed protein function through disruption in enzyme active site.

  Conclusion: Amino acid substitutions as a consequence of COX-2 nsSNPs have important role in human disease. Substitutions which are located in catalytic domain are important for the enzymatic function of COX-2 and associated with higher expression of COX-2.

Keywords: Cancer; Cyclooxygenase-2; Protein Coding Regions; SNP
Full-Text [PDF 198 kb]   (1595 Downloads)    
Type of Study: article | Subject: Special
Received: 2013/01/14 | Accepted: 2013/05/6 | Published: 2013/09/23
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Jahanbakhsh-Godehkahriz S, Naderi E, Mohamadkhani A. Screening of Missense SNPs in Coding Regions of COX-2 as a Key Enzyme Involved in Cancer. J Ardabil Univ Med Sci. 2013; 13 (3) :305-316
URL: http://jarums.arums.ac.ir/article-1-107-en.html


Volume 13, Issue 3 (autumn 2013) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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