Background & objectives: More than 95% of bile ducts diseases are pertain to either gallbladder stone or cholecystitis. Normally, there is not amylase in gallbladder and its presence indicates reflux from the pancreas. The aim of this study was to investigate the association between presence of gallstones and amylase level in gallbladder.

  Methods: In this cross-sectional study 100 patients with cholecystitis were selected and after removal of the gallbladder under sterilized conditions, about 1-5 ml of fluid has been aspirated from bladder. The amylase level of samples was measured in the laboratory and collected data analyzed using statistical tests such as t-test and ANOVA by SPSS 18 software.

  Results : A total of 100 patients with cholecystitis, 42% had measurable amylase in gallbladder (25-65 IU/L). The patients were in the range of 22-92 years old and 47% of them was in the age group of 40-60 years old. The mean age of patients was 48.2 years old (SD=12). There was a significant linear correlation between the gallbladder amylase level and the age of patients (r=0.76, p=0.001).

  Conclusion: Results of our study showed a significant relationship between the formation of gallestones and pancreatic amylase reflux to gallbladder. So, in patients with gallestones, the possible existence of pancreatitis and specially the probability of the pancreas and bile duct obstruction or narrowing should be checked .