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:: Volume 11, Number 2 (summer 2011) ::
J Ardabil Univ Med Sci 2011, 11(2): 134-142 Back to browse issues page
Intervention of Morphine and Naloxone on Analgesic Effects of Origanum vulgare Extract in Male Rat
Yasamin Pahlavan , Gholam Reza Sepehri, Mohammad Reza Afarinesh Khaki, Vahid Sheibani, Khadijeh Esmail Pour Bezenjani, Bahareh Pahlavan
Abstract:   (21645 Views)

 Background and Objectives: Opiodergic system has important role in pain control. Origanum vulgare is a folk medicine with analgesic properties which is widely distributed in the north and northwest parts of Iran. The mechanism of therapeutic effects of Origanum vulgare is not understood. The aim of this study was to evaluate the intervention effect of opioid agonist (morphine) and antagonist (naloxone) on analgesic effects of Origanum vulgare.

 Methods: In this study 28 Male Wistar rats (200-250 g) were used (n=7). The rats were anaesthetized by ketamine (80mg /kg) and xylazine (10mg /kg) and a cannula was inserted into the left ventricle according to atlas of Paxinos characteristics using stereotaxic apparatus. The animals were allowed to recover for 5-7 days .In pilot examination, the effective dose of ORG extract determined 3µg/rat i.c.v. Rats were divided into 4 groups:Control group given saline 0.5ml.i.p/ saline 5µl.i.c.v or ORG 3µg/rat.i.c.v. other groups are morphine (2mg/kg ,i.p) and ORG3µg/rat,i.c.v , Naloxone (1mg/kg,i.p) and ORG 3µg/rat,i.c.v. The latency response of rats to thermal stimulation was recorded (30, 45, 60, 75, 90 &120 min after treatment) by Tail flick test. Repeated Measurement test and ANOVA were used to determine significant differences.

 Results: There was significant decrease in the pain threshold following the co-administration of ORG extract with naloxone in the Tail flick test. There also was significant decrease in the latency response or pain threshold 90 and 120 min after intervention in naloxone group compared with that in control group (p< 0.05).

 Conclusion: The results of this study showed that analgesic effect of aqueous extract of Origanum vulgare may be mediated, at least in part, by opioidergic system.

Keywords: Origanum vulgare Extract; Morphine; Naloxone; Analgesic Effects
Full-Text [PDF 278 kb]   (993 Downloads)    
Type of Study: article | Subject: Special
Received: 2011/03/3 | Accepted: 2011/06/10 | Published: 2011/06/22
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Pahlavan Y, Sepehri G R, Afarinesh Khaki M R, Sheibani V, Esmail Pour Bezenjani K, Pahlavan B. Intervention of Morphine and Naloxone on Analgesic Effects of Origanum vulgare Extract in Male Rat. J Ardabil Univ Med Sci. 2011; 11 (2) :134-142
URL: http://jarums.arums.ac.ir/article-1-182-en.html
Volume 11, Number 2 (summer 2011) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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