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:: Volume 19, Issue 4 (winter 2020) ::
J Ardabil Univ Med Sci 2020, 19(4): 430-438 Back to browse issues page
Detection of Free Living Amoeba Acanthamoeba in the Nasal and Oral Discharges of HIV + / HIV­ Sample in Arak
Omid Ghane Azabadi , Farshideh Didgar , Nader Zarinfar , Fatemeh Rafiei , Zahra Eslamirad
Department of Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, Iran
Abstract:   (587 Views)
 
Background & objectives: Impaired immune system provides favorable conditions for colonization by Acanthamoeba in the human body. In this case control study, we compared the molecular and culture methods in identifying Acanthamoeba in the nasal and oral secretions of HIV+/HIV­ human.
Methods: In a current case control study, nasal and oral discharge of 53, HIV+ patients and 53, HIV­ people were evaluated. The nasal and oral secretions of each patient were prepared by sterile swabs and transferred to the laboratory. All samples were cultured but only the positive samples used for molecular analysis.
Results: By cultivation method, of the 53, HIV+ patients, a total of 11 samples, including 5 nasal and 6 oral samples, were contaminated with Acanthamoeba. Of the 53, HIV­­ people, 3 samples of nasal discharge were contaminated with this parasite. The molecular method approved the contamination of 10 samples, including 5 oral and 5 nasal samples from HIV+ patients with this parasite. Statistical analysis showed the rate of infection in HIV+ patients was significantly different compared to HIV­ people
Conclusion: The results of the current study showed that the rate of Acanthamoeba infection in HIV+ patients was higher than that of HIV- individuals. Also, considering that in the control group (HIV- individuals) only the nasal discharge were infected with the parasite, it seems that in the case group (HIV + patients) the infection of the oral discharge with the parasites is due to the  entry of its cysts into the nose and transmission to the mouth
Keywords: Acanthamoeba, Free-living Amoeba, Nose, Mouth, HIV
Full-Text [PDF 146 kb]   (193 Downloads)    
Type of Study: article | Subject: انگل شناسی- قارچ شناسی- حشره شناسی
Received: 2019/10/22 | Accepted: 2019/12/21 | Published: 2020/06/30
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Ghane Azabadi O, Didgar F, Zarinfar N, Rafiei F, Eslamirad Z. Detection of Free Living Amoeba Acanthamoeba in the Nasal and Oral Discharges of HIV + / HIV­ Sample in Arak. J Ardabil Univ Med Sci. 2020; 19 (4) :430-438
URL: http://jarums.arums.ac.ir/article-1-1781-en.html


Volume 19, Issue 4 (winter 2020) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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