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:: Volume 20, Issue 2 (summer 2021) ::
J Ardabil Univ Med Sci 2021, 20(2): 242-253 Back to browse issues page
Mitochondrial/Lysosomal Protective and Antioxidant Agents Reduce Toxicity Induced by Toluene in Human Lymphocytes
Enayatollah Seydi , Jalal Pourahmad , Behnaz Shoja Talatappe , Ahmad Salimi
Department of Pharmacology and Toxicology, School of Pharmacy, Ardabil University of Medical Science, Ardabil, Iran
Abstract:   (206 Views)
 
Background & objectives: Toluene as a systemic toxin and industrial solvents has different effects on vital organs of the body. There is little mechanistic study of the interactions between toluene and human lymphocytes. In this study, the direct toxicity of toluene and the potential of agents with antioxidant, mitochondrial/lysosomal protective effects to reduce its possible toxicity in human lymphocytes were studied.
Methods: Blood lymphocytes were isolated from healthy male volunteerchr('39')s blood, using Ficoll Paque Plus followed by gradient centrifugation. In this study, cell viability, reactive oxygen species (ROS) level, lipid peroxidation (LPO), mitochondrial membrane potential (MMP), lysosomal membrane damage, glutathione (GSH) and glutathione disulfide (GSSG) levels, were determined in blood lymphocytes after incubation with toluene and antioxidant, mitochondrial and lysosomal protective compounds.
 Results: Results showed that toluene reduced lymphocyte viability, increased ROS levels, LPO content, damage to lysosomal membranes, mitochondrial damages and GSH depletion, which these damages were significantly inhibited by dibutyl hydroxytoluene (BHT) as a synthetic antioxidant, cyclosporine A (Cs. A) as an inhibitor of mitochondrial pores, and chloroquine as a lysosomotropic agent.
Conclusion: Results of our study suggest that using of antioxidants, mitochondrial and lysosomal protective agents can be effective in reducing toluene-induced toxicity in exposed individuals.
Keywords: Toluene, Antioxidant, Cytotoxicity, Mitochondria, Mitochondrial Protective Agents
Full-Text [PDF 1847 kb]   (75 Downloads)    
Type of Study: article | Subject: بهداشت
Received: 2020/06/21 | Accepted: 2021/01/29 | Published: 2021/01/29
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Seydi E, Pourahmad J, Shoja Talatappe B, Salimi A. Mitochondrial/Lysosomal Protective and Antioxidant Agents Reduce Toxicity Induced by Toluene in Human Lymphocytes. J Ardabil Univ Med Sci. 2021; 20 (2) :242-253
URL: http://jarums.arums.ac.ir/article-1-1881-en.html


Volume 20, Issue 2 (summer 2021) Back to browse issues page
مجله دانشگاه علوم پزشکی اردبیل Journal of Ardabil University of Medical Sciences
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